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20 µg
蛋白名称:Cystatin F/CST7蛋白, Cystatin F/CST7 protein
蛋白构建:A DNA sequence encoding the mouse CST7 (O89098) (Met1-Gln144) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 93 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008. The IC50 is < 6 nM.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 19
蛋白分子量:The recombinant mouse CST7 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 367 amino acids and has a predicted molecular mass of 41.4 KDa. The apparent molecular mass of the protein is approximately 42 KDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:O89098
蛋白氨基酸序列:Met1-Gln144
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验ROTORS Table F.1. Rotor Characteristics Figure F.1. Cross section of Sorvall SS-34 rotor. Rotors for a centrifuge are either fixed angles , swinging buckets , continuous flow, or zonal, depending upon whether the sample is held
Ffactor (F 因子):细菌性或繁殖质粒。 F1 generation (F1代):两个亲本系(同源)杂交后的第一代。 Facultative heterochromatin (兼并性异染色质):指同时存在活泼型拷贝的惰性序列。如,哺 乳动物雌性中的一条X 染色体。 Fast component (快速变性区):复性反应中的快成分是首先复性,含有高重复DNA的成分。 Fate map (原基分布图):在胚胎上标示该区域内细胞的后代将会发育而成的成熟组织。
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
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