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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human NGF / NGFB Protein (Catalog#11050-HNAC)
- 亚型:
Mouse IgG1
- 形态:
液体
- 保存条件:
在-20℃ to -80℃
- 克隆性:
MAb
- 适应物种:
鼠
- 保质期:
12个月
- 抗原来源:
11050-HNAC
- 目录编号:
11050-MM06
- 级别:
免疫学试剂
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 宿主:
Mouse
- 应用范围:
ELISA
- 浓度:
ELISA: 1:1000-1:2000
- 靶点:
NGF
- 抗体英文名:
NGF / NGFB Antibody, Mouse MAb
- 抗体名:
NGF / NGFB 鼠单抗
- 规格:
20 µL/100 µL
| 规格: | 20 µL | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 100 µL | 产品价格: | ¥1699.0 |
NGF / NGFB 鼠单抗(产品说明)
抗体种属:Human
抗体靶点:NGF
抗体应用:ELISA
抗原货号:11050-HNAC
抗原描述:Recombinant Human NGF / NGFB Protein (Catalog#11050-HNAC)
抗体宿主:Mouse
抗体Ig类型:Mouse IgG1
抗体纯化方法:Protein A
抗体制备:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NGF / NGFB (rh NGF / NGFB; Catalog#11050-HNAC; NP_002497.2; Ser122-Arg239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
抗体特异性:Human NGF / NGFB
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验1, Wang, J; et al. Involvement of alpha-7 nicotinic receptor (α7nAChR) in respiratory syncytial virus infection-induced asthma in rats. Research Square
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
to the SAMPLE BUFFER to increase the amount of NGF removed from the sample. Typically, a 10x dilution (w/v) of tissue samples will give a signal sufficient for detection. Once diluted, samples are homogenized on ice for 30 seconds using a cell sonicator at micro
test 每只大鼠取双侧海马CA3区DG区切片各两张,在计算机图像分析系统(Meta Morph/Ax70)上测量NGF、NT3 平均光密度和平均目标灰度值(所测阳性细胞平均灰度),灰度单位分为256个等级,免疫反应的强弱与灰度值呈反比关系。 采用SPSS for Windows 10.0软件分析,数据以均数加减标准差(x- ±s)表示,组间检验采用t检验,p值取0.05。porsolt swim test (责任编辑:admin)
慢性应激对大鼠海马NGF、NT-3表达的影响及应激停止后的变化
摘要 :目的 探讨慢性应激对大鼠海马神经元NGF、NT-3蛋白表达的影响及其应激停止后的变化。方法 采用慢性强迫游泳制作动物模型。运用open-field法观察大鼠行为学的变化,运用免疫组织化学方法观察大鼠海马DG区、CA3区NGF、NT-3的表达。结果与对照组相比,实验组1大鼠海马CA3、DG区NGF、NT-3的平均灰度值显著增加(p 关键词 :慢性应激 海马 NGF、NT-3 强迫游泳porsolt swim test
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