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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within human GAPDH.
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆
- 标记物:
见下方详细说明
- 适应物种:
H, P
- 保质期:
1-2年
- 抗原来源:
KLH-conjugated synthetic peptide encompassing a sequence within human GAPDH.
- 目录编号:
251330
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Rabbit
- 应用范围:
E, WB, IHC, IP
- 浓度:
见详细说明信息
- 靶点:
GAPDH
- 抗体英文名:
GAPDH Antibody
- 抗体名:
GAPDH Antibody
- 规格:
0.1 mg
GAPDH Antibody产品详细介绍:
| 产品名称: | GAPDH Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 251330 |
| 规格: | 0.1 mg |
| 产品描述: | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key emzymes in glycolysis. GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). GAPDH is also involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH is constitutively expressed at high level in almost all tissue and cell lines making an ideal positive control marker in immunotechniques. This antibody works best for ELISA and immunohistochemistry. |
| 克隆类型: | Rabbit Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | KLH-conjugated synthetic peptide encompassing a sequence within human GAPDH. |
| 适用物种: | H, P |
| 应用范围: | E, WB, IHC, IP |
| 应用说明: | E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500; IP: 1:100-1:500 |
| 别名: | Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
ABBIOTEC产品提供质量保证,提供完善的售后和技术支持,ABBIOTEC产品正品包装:
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文献和实验GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
,但是进口品牌如Abcam、Millipore、CST等价格偏高,一般在¥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体,分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody (货号E021010 )是小鼠源的单抗,其效价很高,厂商推荐的WB起始稀释比率为1:1000,实际应用过程中,有使用1:10000或更高的报告,远远高于此稀释比率,说明其免疫效价很高,另外,其检测种属也很多,包括人、牛、猪、小鼠
【信息】Rabbit anti-GAPDH(SF-PA005)(兔抗 GAPDH 多克隆 IgG 抗体)招代理
Rabbit anti-GAPDH(SF-PA005)(兔抗 GAPDH 多克隆 IgG 抗体) 背景 甘油醛 -3- 磷 酸脱氢酶( Glyceraldehyde 3-phosphate dehydrogenase,GAPDH)是糖酵解(glycolysis)过程中的关键酶,分子量为37kDa 左右。它催化甘油醛-3-磷酸的氧化磷酸化反应。GAPDH 作为看家基因 在同种细胞 或者组织中一般是恒定、高水平表达的,常用作 Western Blot 实验的内参照. 来源 兔抗
在免疫反应方面,基于抗原的刺激在体内形成、并与抗原作特异性结合的蛋白质之总称。其化学实质是免疫球蛋白。抗体,按照抗原抗体反应的类型可分别称为沉淀素、凝集素、溶血素、溶菌素、抗毒素、中和抗体等,但这些并不等于有相应的不同抗体存在,一般多只表明抗体反应的多样性而已。抗体在血清中含量最多(体液抗体=humoral antibody);含有某特定抗体的血清称为抗血或免疫血清。
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