- ￥800 - 1200
- 爱必信(absin)
- 上海
- abs130609
- 2025年07月09日
- WB: 1:5000~1:50000, ELISA(peptide) 1:20000-1:40000
- Rabbit
- -
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human GAPDH.
- 亚型：
-
- 形态：
-
- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 克隆性：
-
- 标记物：
-
- 适应物种：
-
- 保质期：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 抗原来源：
-
- 目录编号：
-
- 级别：
-
- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB: 1:5000~1:50000, ELISA(peptide) 1:20000-1:40000
- 浓度：
1mg/ml
- 靶点：
-
- 抗体英文名：
Rabbit anti-GAPDH Polyclonal Antibody
- 抗体名：
GAPDH抗体
- 规格：
100ug/50ug
| 规格： | 100ug | 产品价格： | ￥1200.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥800.0 |
| 概述 | |
| 描述 | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types. |
| 别名 | GAPDH抗体 |
| 宿主 | Rabbit |
| 特异性 | GAPDH antibody detects endogenous levels of total GAPDH protein from human and monkey.Is unsuitable for MOUSE and RAT,not yet tested in other species. |
| 反应种属 | Human, Monkey |
| 预测反应种属 | Zebrafish;Rabbit;Pig;Dog;Horse |
| 应用 | WB: 1:5000~1:50000, ELISA(peptide) 1:20000-1:40000 |
| 分子量 | Predicted molecular weight: 36kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 | A synthesized peptide derived from human GAPDH. |
| 性能 | |
| 浓度 | 1mg/ml |
| 纯化方法 | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |
| 类型 | Polyclonal Antibody |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| 存储溶液 | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 靶标 | |
| 背景说明 | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. |
| 翻译后修饰 | S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.ISGylated.Sulfhydration at Cys-152 increases catalytic activity.Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation. |
| 细胞定位 | Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane; |
| UniProt | P04406 |
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