- ￥2262
- LSM Bio
- 进口
- PAab03342
- 2025年07月17日
- ELISA,IHC,WB,IP,IF,FC
- E. coli - derived recombinant protein
- Human,Mouse ,Rat,Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
glyceraldehyde-3-phosphate dehydrogenase
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse ,Rat,Pig
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
P15248
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,IHC,WB,IP,IF,FC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
glyceraldehyde-3-phosphate dehydrogenase
- 抗体英文名：
anti-GAPDH antibody,GAPDH antibody
- 抗体名：
anti-GAPDH 抗体,GAPDH 抗体
- 规格：
100μg
GAPDH抗体| GAPDH antibody
货号 PAab03342
蛋白别名 GAPD
蛋白介绍
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
产品描述
anti-GAPDH antibody is a Rabbit Polyclonal antibody againstGAPDH..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human breast cancer using PAab03342(GAPDH antibody) at dilution of 1:100
Western blot
Western blot of Hela cell with anti-GAPDH (PAab03342) at various dilutions. PAab03342(本抗体仅供体外科研用途，不可用于临床诊断!) <"">
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文献和实验GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
，但是进口品牌如Abcam、Millipore、CST等价格偏高，一般在￥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体，分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody （货号E021010 ）是小鼠源的单抗，其效价很高，厂商推荐的WB起始稀释比率为1:1000，实际应用过程中，有使用1:10000或更高的报告，远远高于此稀释比率，说明其免疫效价很高，另外，其检测种属也很多，包括人、牛、猪、小鼠
【信息】Rabbit anti-GAPDH(SF-PA005)（兔抗 GAPDH 多克隆 IgG 抗体）招代理
Rabbit anti-GAPDH(SF-PA005)（兔抗 GAPDH 多克隆 IgG 抗体） 背景 甘油醛 -3- 磷 酸脱氢酶（ Glyceraldehyde 3-phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶，分子量为37kDa 左右。它催化甘油醛-3-磷酸的氧化磷酸化反应。GAPDH 作为看家基因 在同种细胞 或者组织中一般是恒定、高水平表达的，常用作 Western Blot 实验的内参照. 来源 兔抗
在免疫反应方面，基于抗原的刺激在体内形成、并与抗原作特异性结合的蛋白质之总称。其化学实质是免疫球蛋白。抗体，按照抗原抗体反应的类型可分别称为沉淀素、凝集素、溶血素、溶菌素、抗毒素、中和抗体等，但这些并不等于有相应的不同抗体存在，一般多只表明抗体反应的多样性而已。抗体在血清中含量最多（体液抗体=humoral antibody）；含有某特定抗体的血清称为抗血或免疫血清。
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