重组人白介素-3（rHuIL-3）

Recombinant Human Interleukin 3 

品名	rHuIL-3
规格	10μg、100μg
描述	含133个氨基酸，理论分子量为15.1kD，有糖基化位点。
来源	酵母。
分子量	约为15-25kD左右的糖蛋白。
纯度	≥97%。
内毒素含量	<1EU/µg，用鲎试剂法进行测定。
活性	用细胞增殖的方法进行测定，以TF-1为细胞依赖株。比活性≥1.8×106 IU/mg。
配方	本品为无菌冻干粉，缓冲体系为PBS，pH7.4。
复溶	建议开盖前以10,000rpm离心30-60秒，或3,000rpm离心5分钟，再使用0.2ml无菌水复溶。
保存	冻干样品自生产之日起在-20℃到-70℃可以稳定保存36个月。 溶解以后，该细胞因子能在2-8℃的无菌条件下保存一个月，或在-20℃到-70℃的人工除霜的冰箱中保存三个月，而无明显活性损失。 避免反复冻融。

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-­3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-­cell stimulating factor, burst promoting activity, multi­-colony stimulating factor, thy­-1 inducing factor and WEHI­-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL­-3. At the amino acid sequence level, mature human and murine IL­-3 share only 29% sequence identity. Consistent with this lack of homology, IL­-3 activity is highly species-­specific and human IL-­3 does not show activity on murine cells. IL­-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-­3 signaling is composed of at least two subunits, an IL­-3 specific α chain which binds IL-­3 with low affinity and a common β chain that is shared by the IL-­5 and GM­-CSF high affinity receptors. Although the β chain itself does not bind IL­-3, it confers high ­affinity IL-­3 binding in the presence of the α chain. Receptors for IL­-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.
仅供实验室使用。