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生物学活性: |
- 用IL-1alfa依赖性细胞株D10.G4.1细胞增殖的方法进行测定,比活性≥1.6x108IU/mg。
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配方: |
- 本品为无菌冻干粉,缓冲体系为PBS, pH7.4,0.1%HSA。
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保存: |
- 冻干样品自生产之日起在-20℃到-70℃可以稳定保存36个月。
- 溶解以后,该细胞因子能在2-8℃的无菌条件下保存一个月,或在-20℃到-70℃的人工除霜的冰箱中保存三个月,而无明显活性损失。
- 避免反复冻融。
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Interleukin 1 (IL-1) is a name that designates two proteins, IL-1α and IL-1β, which are the products
of distinct genes, but which show approximately 25% amino acid sequence identity and which
recognize the same cell surface receptors. Although IL-1 production is generally considered to be
a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated
during bone formation and the menstrual cycle and can be induced in response to nervous system
stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial
endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells
is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages,
keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells,
fibroblasts and glia (oligodendroglia, astrocytes and microglia). IL-1α and IL-1β are both
synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular
weights of approximately 18,000 Da. Neither precursor contains a typical hydrophobic signal
peptide sequence and most of the precursor form of IL-1α remains in the cytosol of cells, although
there is evidence for a membranebound form of the precursor form of IL-1α. The IL-1α precursor
reportedly shows full biological activity in the EL 4 assay. Among various species, the amino acid
sequence of mature IL-1α is conserved 60% to 70% and human IL-1 has been found to be
biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated
type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction
and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of
IL-1 to the type I receptor.
仅供实验室使用。