Phospho-TACC3 (Ser558) Antibod

5645:

Western Blotting

Phospho-TACC3 (Ser558) Antibody detects endogenous levels of TACC3 protein only when phosphorylated at Ser558.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser558 of human TACC3 protein. Antibodies are purified by Protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HT-29 and U-2 OS cells, untreated or synchronized in mitosis by thymidine block (2 mM, 16 hours) followed by nocodozole treatment (100 ng/ml, 24 hours), using Phospho-TACC3 (Ser558) Antibody.

Transforming acid coiled-coil (TACC) proteins are a family of proteins characterized by a common coiled-coil motif of approximately 200 amino acids at the carboxy-terminal end (1). Three family members have been identified in humans: TACC1, TACC2, and TACC3. These proteins are thought to be involved in centrosomal microtubule assembly and have been mapped to chromosomal regions that are disrupted in some cancers (reviewed in 2). TACC3 has been shown to be upregulated in many cancer cell lines (3). When phosphorylated at Ser558 by Aurora A, mammalian TACC3 is localized to mitotic spindles and increases microtubule stability (4,5). For this reason, it has been suggested that monitoring the localization of phosphorylated TACC3 would be an effective way to determine the efficacy of Aurora A inhibitors that show promise as anti-cancer drugs (6,7). In addition, studies have shown that TACC3 could be useful as a prognostic marker for non-small cell lung cancer (8).

1. Gergely, F. et al. (2000) Proc Natl Acad Sci USA 97, 14352-7.
2. Peset, I. and Vernos, I. (2008) Trends Cell Biol 18, 379-88.
3. Still, I.H. et al. (1999) Genomics 58, 165-70.
4. Kinoshita, K. et al. (2005) J Cell Biol 170, 1047-55.
5. Schneider, L. et al. (2007) J Biol Chem 282, 29273-83.
6. LeRoy, P.J. et al. (2007) Cancer Res 67, 5362-70.
7. Tyler, R.K. et al. (2007) Cell Cycle 6, 2846-54.
8. Jung, C.K. et al. (2006) Pathol Int 56, 503-9.

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For Research Use Only. Not For Use In Diagnostic Procedures.