- 询价
- Cell Signaling Technology
- 9566
- USA
- 2025年11月03日
- W, IP
- Rabbit
- H,M,R,C,X,Z
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-β-Catenin (Ser552) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser552 of human β-catenin
- 应用范围：
W, IP
- 宿主：
Rabbit
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,R,C,X,Z
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat C=Chicken X=Xenopus Z=Zebrafish
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M (R) (C) (X) (Z) | Endogenous | 92 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-β-Catenin (Ser552) Antibody detects endogenous levels of β-catenin only when phosphorylated at Ser552. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human β-catenin. Antibodies are purified by peptide affinity chromatography. Western Blotting
Western blot analysis of total cell lysates from SK-N-MC cells, treated with forskolin (FSK) for 30 minutes, or the lysate was treated with λ phosphatase for 1 hour, using Phospho-β-Catenin (Ser552) Antibody (upper) or β-Catenin Antibody (Amino-terminal Antigen) #9581 (bottom). Western Blotting
COS-7 cells were transfected with cDNAs for the DYKDDDDK-tagged wild type (WT) β-catenin or for Ser-to-Ala β-catenin mutants as indicated. Cells were stimulated with 10 mM forskolin (FSK) for 5 minutes and lysed. β-catenin or its mutants were immunoprecipitated with DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 and analyzed by western blotting with Phospho-β-Catenin (Ser552) Antibody #9566, Phospho-β-Catenin (Ser675) Antibody #9567, or DYKDDDDK Tag Antibody as indicated (Figures provided by Drs. Sebastien Taurin and Nickolai Dulin, Department of Medicine / Pulmonary, The University of Chicago). |
| Background | β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). Both Akt and PKA were shown to phosphorylate β-catenin at Ser552. Phosphorylation at Ser552 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9-11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Anti-FLAG® is a registered trademark of Sigma-Aldrich. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
dongreyuan 有的抗体名称上面写着Ab-33，比如，β-catenin（Ab-33）antibody，请问，Ab-33是什么意思？ bettyzyj 这个抗体是非磷酸化的抗体non-phospho-antibody ，33 代表的是基因位点 dongreyuan 谢谢了 yhwangcams bettyzyj wrote
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