- 询价
- Cell Signaling Technology
- 2555
- USA
- 2025年08月06日
- W, IHC-P
- Rabbit
- All
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
GFP Antibody
- 抗原：
synthetic GFP peptide
- 应用范围：
W, IHC-P
- 宿主：
Rabbit
- 适应物种：
All
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | All | Transfected Only | 27 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | GFP Antibody detects GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27 kDa to the molecular weight of the fusion protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic GFP peptide. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from COS-7 cells, mock transfected (lane 1) or expressing GFP-tagged fusion protein (lane 2), using GFP Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HCC827 cells control (left) or GFP-transfected (right) using GFP Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded normal mouse lung (left) and C57BL/6-Tg(CAG-EGFP)10sb/J mouse lung (The Jackson Laboratory) (right) using GFP Antibody. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体？
对照，2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R]（货号#E022030），其免疫原是KLH偶联的水母GFP蛋白的N末端多肽，由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致，因此从免疫原上来说，GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是，EarthOx对于该单克隆抗体还进行了明确的验证试验，试验也表明该Anti-GFP Tag Monoclonal
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naj2007 请教一下各位老师，在构建过表达载体的时候，VEGF和GFP是不是不可以做成融合蛋白，但是如果用不同的启动子的话，很容易丢失荧光，有没有什么解决方法。 zhujoker 可以做成融合蛋白的，即使是使用不同的启动子的话荧光也不那么容易丢失的，大不了你将荧光的启动子换成强的如CMV，EF1a之类。 kinguangjun 或者你可以用CMV-VEGF-IRES-GFP来做
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