- 询价
- Cell Signaling Technology
- 2781
- USA
- 2025年07月11日
- W
- Rabbit
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-FADD (Ser194) Antibody (Human Specific)
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser194 of human FADD
- 应用范围：
W
- 宿主：
Rabbit
- 适应物种：
H
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 28 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-FADD (Ser194) Antibody (Human Specific) detects endogenous levels of human FADD protein only when phosphorylated at serine 194. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser194 of human FADD. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from Jurkat and HeLa cells, hydroxyurea-treated (4 mM, 20 hours) or nocodazole-treated (1 µg/ml, 20 hours), using Phospho-FADD Ser194 Antibody (Human Specific) (upper) or control FADD Antibody (Human Specific) #2782 (lower). |
| Background | Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase-8 (1,2). FADD has a carboxy-terminal death domain, which interacts with the cytoplasmic tail of the membrane receptor, and an amino-terminal death effector domain, which interacts with caspase-8. Clustering of the receptors upon stimulation brings about FADD and caspase-8 oligomerization, activating the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle-dependent (3) and may be related to its regulatory role in embryonic development and cell cycle progression (4,5). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation
The reversible phosphorylation of proteins is a key mechanism whereby signalling cascades involved in the response to extracellular stimuli bring about changes in cellular function. These proteins include the kinases/phosphatases that form
Generation and Application of Phospho-specific Antibodies for p53 and pRB
of phosphoproteins. We have improved methods for generation of phosphorylation site-specific antibodies and have successfully obtained antibodies for the analysis of most of the phosphorylation sites on p53 and RB proteins.
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