- 询价
- Cell Signaling Technology
- 5880
- USA
- 2026年05月29日
- W, IP, IHC-P
- H,B
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- 文献和实验
- 技术资料
- 抗体英文名：
eNOS (6H2) Mouse mAb
- 抗原：
recombinant protein specific to human eNOS protein
- 应用范围：
W, IP, IHC-P
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
H,B
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H B | Endogenous | 140 | Mouse IgG1 |
| Protocols | |
|---|---|
| Specificity / Sensitivity | eNOS (6H2) Mouse mAb recognizes endogenous levels of total eNos protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human eNOS protein. Western Blotting
Western blot analysis of extracts from BAEC and HUVEC cells using eNOS (6H2) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using eNOS (6H2) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using eNOS (6H2) Mouse mAb. |
| Background | Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2 O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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