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- Cell Signaling Technology
- USA
- 6346S
- 2026年01月22日
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文献和实验Inducing RNAi with siRNA Cocktails Generated by RNase III
products (21 bp range) can effectively mediate RNAi in both mammalian cells (10) and mouse embryos (11). We demonstrate here that the siRNA cocktails produced by complete digestion by RNase III (~12 to 15 bp) are capable of silencing specific genes
eliminate the guesswork--and the tedious labwork--associated with siRNA design and testing. Applied Biosystems' gene-specific, ready-to-run TaqMan® Gene Expression Assays provide you with a fast, simple way to measure the efficacy of mRNA knockdown
Isolate It All：siRNA • miRNA • Total RNA • Native Protein
[1] demonstrated a correlation between loss of 5S rRNA and tRNA, and inefficient recovery of miRNA or siRNA (see also Figure 3 and 6) using standard GFF protocols. The integrity of the isolated total RNA was assessed on an RNA 6000 Nano LabChip® with an Agilent
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