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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试,看看是否成功免疫。
5、如果成功免疫,杀死实验动物,采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
ELISA夹心法测定中,HA与试剂抗体(捕获抗体和标记抗体)的结合,可通过Fc-Fc 和Fab-Fab两种结合方式干扰测定。正常情况下,在ELISA 夹心法中,待测抗原同时与捕获抗体和标记抗体结合,EGF Receptor (EGFR1) Mouse mAb (IP specific)形成了固相捕获抗体-待测抗原-标记抗体的复合物(如图1A所示)。在以Fc-Fc 的方式结合的机制中,HA的Fc 端结合到捕获抗体的Fc 段上,而HA的Fab 区域则结合到标记抗体的Fab 区域上,导致假阳性结果(如图1B所示)。在以Fab-Fab 结合方式的机制可引起假阳性或假阴性的结果,如HA的Fab区域通过分别结合捕获抗体的Fab区域和标记抗体的Fab 区域,EGF Receptor (EGFR1) Mouse mAb (IP specific)阻挡捕获抗体和标记抗体与待测抗原的结合,从而导致假阴性(如图1C所示);HA的F(ab’)2 通过同时结合捕获抗体的Fab区域和标记抗体的Fab区域,形成固相捕获抗体-HA-标记抗体复合物,如果标本中无待测抗原,HA就引起假阳性的结果。
EGF Receptor (EGFR1) Mouse mAb (IP specific):xy-0613xy Eukaryotic aspartyl protease(Rice)天冬氨酸蛋白酶家族蛋白(水稻蛋白的一种)(抗原)
xy-0711xy FAM3B/PANDER(PANcreatic DERived factor)一种新型的胰岛细胞因子(抗原)
xy-0788xy Fabp2 (fatty acid binding protein 2, intestinal)脂肪酸结合蛋白2(抗原)
xy-0500xy ECG2 (sophagus cancer-related gene-2)食道癌相关基因(抗原)
xy-0666xy Fibronectin (FN)纤维连结蛋白(多肽抗原)
xy-0734xy FGF-7/KGF (Keratinocyte growth factor precursor)成纤维细胞生长因子-7/纤维母细胞生长因子 (抗原)
xy-0675xy FGFR2(Fibroblast Growth Factor Receptor 2)成纤维细胞生长因子受体2抗原
xy-0735xy FGF-8/HBGF-8 (Fibroblast growth factor 8)成纤维细胞生长因子-8/纤维母细胞生长因子-8 (抗原)
xy-0400xy GAD-65 (glutamate decarboxylase)谷氨酸脱羧酶-65(C端多肽)
xy-0721xy Galectin-3 半乳糖凝集素-3(抗原)
xy-0701xy GCS (glucosylceramide synthase, GCS)葡萄糖神经酰胺合成酶(抗原)
xy-0639xy GFP绿色荧光蛋白
xy-0725xy ER-alpha雌激素受体alpha
xy-0654xy GHR (growth hormone receptor)生长激素受体(抗原)
xy-0472xy GLUT1(Glucose transporter type 1)葡萄糖转运蛋白1(抗原)
xy-0618xy hypothetical protein (LOC55652)hypothetical protein 抗原
xy-0402xy HCCR-1(BRI3 binding protein)人宫颈癌基因/bri3结合蛋白(抗原)
xy-0343xy pre S1/S2 protein [HBV]乙肝病毒pre S1/乙肝病毒pre S2(抗原)
xy-0728xy HCV-HCBP1/ACY-3(Hepatitis C virus core-binding protein 1)丙型肝炎病毒核心蛋白结合蛋白-1(抗原)
xy-0746xy HHV8/ORF50(Human herpesvirus 8 type P)人类疱疹病毒8抗原
xy-0869xy HHV8/ORF K2/vIL-6(Human herpesvirus 8)人类疱疹病毒8抗原
xy-0737xy HIF-1Alpha缺氧诱导因子1α 多肽
xy-0483xy histone H3-like protein组蛋白H3样蛋白多肽
xy-0620xy histone H3-like protein组蛋白H3样蛋白(C端多肽)
xy-0621xy histone H3-like protein组蛋白H3样蛋白(N端多肽)
xy-0785xy HIV-1(human immunodeficiency virus type 1)艾滋病病毒-1/人类免疫缺陷病毒1型(抗原)
xy-0786xy HIV-1(human immunodeficiency virus type 1)艾滋病病毒-1/人类免疫缺陷病毒1型(抗原)
xy-0887xy Measles virus protein(Plant2 derived measles virus hemagglutinin protein)麻疹病毒红血球凝集素蛋白多肽
xy-0888xy Measles virus protein(Plant2 derived measles virus hemagglutinin protein)麻疹病毒红血球凝集素蛋白多肽
xy-0455xy HSL(hormone-sensitive lipase)荷尔蒙敏感脂肪酸(抗原)
xy-0730xy HSP-27(Heat Shock Protein 27)热休克蛋白-27(抗原)
xy-0696xy HSP-90Alpha(Heat Shock Protein 90Alpha )热休克蛋白90α(抗原)
xy-0135xy HSP-90 Beta (heat sock protein-90 Beta)热休克蛋白-90β (抗原)
xy-0284xy IASPP(inhibitory member of the ASPP family)肿瘤细胞凋亡ASPP家族的另一个成员(抗原)
xy-0608xy CD54/ICAM-1细胞间粘附分子-1
xy-0812xy IL-1 Beta(Interleukin-1 Beta)白介素1β(白细胞介素-1β)(多肽抗原)
xy-0445xy IL1RAPL1 (Interleukin 1 receptor accessory protein-like 1)白介素受体相关蛋白样1前体蛋白(抗原)
xy-0379xy IL-6(Interleukin-6)白介素6(白细胞介素-6)(多肽抗原)
xy-0698xy IL-10(Interleukin-10)白介素10(白细胞介素-10)(抗原)
EGF Receptor (EGFR1) Mouse mAb (IP specific)单抗的最大优势是它的特异性、均一性、高效性和无限供应性。在免疫学、医学、生物学等领域的基础研究和临床医学上,包括对疾病(包括癌症)的诊断、预防和治疗等方面,均显示出巨大的生命力。
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文献和实验A Historical Perspective of the EGF Receptor and Related Systems
and physiological role that EGF plays during development and in adult animals led to the identification of its receptor (EGFR) as well as the other members of the EGF family of growth factors and their receptors. In this chapter we provide a historical overview
Assays to Monitor Degradation of the EGF Receptor
to conduct receptor downregulation assays are described. Monitoring of ubiquitination and degradation of the EGFR subsequent to stimulation with the receptor ligand EGF is described. Finally, protocols to quantitatively measure degradation of the EGFR
Motility Signaled From the EGF Receptor and Related Systems
Cell motility is now recognized as central to many biological processes. Growth factors, such as those that activate the epidermal growth factor receptor (EGFR), drive biochemically and biologically distinct subsets of migration critical
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