Stemgent® Phycoerythrin (PE) a

Stemgent® Phycoerythrin (PE) anti-Human TRA-1-60 Antibody详细信息：
Product Overview


The TRA-1-60 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed on undifferentiated human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

Stemgent® Phycoerythrin (PE) anti-Human TRA-1-60 AntibodyProduct Specifications

Imunogen Human embryonal carcinoma (EC) cell line 2102Ep
Reactivity Human
Clone TRA-1-60
Isotype Mouse IgM, κ
Number of Tests 50
Formulation Phosphate-buffered solution, pH 7.2, containing ≤0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and free antibody.
Storage Store undiluted at 4°C. Do not freeze.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by flow cytometry and immunocytochemistry to ensure product quality.
Recommended Usage 20 μl (0.25 μg) per 1x106 viable cells.
General Protocol for Immunofluorescent Staining Using Flow Cytometry
Isolate and dissociate cells to a single cell suspension. Collect cells in a 15 ml conical tube.
Centrifuge the cell suspension at 300 x g for 5 minutes at 4°C. Resuspend the cell pellet to a concentration of 0.2x107 to 1x107 cells per ml in PBS. Keep cells on ice.
Stemgent® Phycoerythrin (PE) anti-Human TRA-1-60 AntibodyFor each sample, add 100 μl of the cell suspension to a 4 ml roundbottom tube and add the appropriate amount (10-20 μl) of the purified antibody.
Incubate at 4°C for 30 minutes to 1 hour.
Add PBS to bring cell suspension to 4 ml. Centrifuge at 300 x g for 5 minutes at 4°C.
Aspirate and agitate the cell pellet. Add 10 &muL (0.25 &mug) of 7- Aminoactinomycin D (cell viability dye) to each sample and incubate for 20 minutes at 4°C.
Add appropriate volume of PBS to each tube. The cells are ready to be analyzed by flow cytometry.
Notice to Purchaser Purchaser represents and warrants that it will use Phycoerythrin (PE) antihuman SSEA-4 purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Stemgent® Phycoerythrin (PE) anti-Human TRA-1-60 Antibody
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