Description |
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Polyclonal Antibody against Pax-6 |
Intended Use |
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**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
Form |
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Purified Antibody (Stored in PBS + 0.03% Thimerosal) |
Host |
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Rabbit |
Species Reactivity |
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Extensive |
[Ab] |
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Pax-6 Polyclonal Antibody, Purified 2 mg/mL |
Specificity |
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This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein. The sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a Protein A column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes. |
Uses |
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This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunofluorescence (IF) and staining of tissue sections. |
Suggested Working Dilution |
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The optimal working dilution should be determined for each specific assay condition.
- Western blot: 1:200-500*
- IHC: 1:300
- IF: 1:300
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Notes |
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*Predicted MW = 47-50 kDa |
Storage |
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Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
References |
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Pax-6 Polyclonal Antibody, Purified Marquardt T, Ashery-Padan R, Andrejewski N, Scardigli R, Guillemot F, Gruss P. Pax6 is required for the multipotent state of retinal progenitor cells. Cell 105(1):43-55, 2001. Yamamoto Y, Jeffery WR. Central role for the lens in cave fish eye degeneration.Science 289(5479):631-633, 2000. Ashery-Padan R, Marquardt T, Zhou X, Gruss P. Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye. Genes Dev 14(21):2701-2711, 2000. Osumi N, Hirota A, Ohuchi H, Nakafuku H, Iimura T, Kuratani S, Fujiwara M, Noji S, Eto K. Pax-6 is involved in the specification of hindbrain motor neuron subtype.Development 124:2961-2972, 1997. Koroma BM, Yang JM, Sundin OH. The Pax-6 homeobox gene is expressed throughout the corneal and conjunctival epithelia. Invest. Ophthalmol Vis Sci 38:108-120, 1997. Davis JA, Reed RR. Role of Olf-1 and Pax-6 transcription factors in neurodevelopment. J Neurosci 16:5082-5094, 1996. [IHC] |
Warranty/Conditions |
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Covance products may not be resold or modified for resale without prior written approval. |