Cdc42 Activation Assay Kit

Product Uses Include

Cdc42 Activation Assay Kit Introduction
The Rho switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of the GTPases is of obvious biological significance and is a subject of intense investigation. The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Rac and Cdc42 protein activation. The assay uses the Cdc42/Rac Interactive Binding (CRIB) region (also called the p21 Binding Domain, PBD) of the Cdc42 / Rac effector protein, p21 activated kinase 1 (PAK). The CRIB/PBD protein motif has been shown to bind specifically to the GTP-bound form of Rac and/or Cdc42 proteins. The fact that the PBD region of PAK has a high affinity for both GTP-Rac and GTP-Cdc42 and that PAK binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of both Rac and Cdc42 make it an ideal tool for affinity purification of GTP-Rac and GTP-Cdc42 from cell lysates. The PAK-PBD protein supplied in this kit corresponds to residues 67-150. This includes the highly conserved CRIB region (aa 74-88) plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42. The PAK-PBD is in the form of a GST fusion protein, which allows one to "pull-down" the PAK-PBD/GTP-Cdc42 (or GTP-Rac) complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Rac/Cdc42 activation in cells. The amount of activated Cdc42 is determined by a Western blot using a Cdc42 specific antibody.
Cdc42 Activation Assay Kit
xy-0191xy HSP-60( Heat shock protein-60)热休克蛋白-60（抗原）
xy-0126xy HSP-70 peptide热休克蛋白-70（多肽抗原）
xy-1228xy alpha Adaptin/AP1衔接蛋白α抗原
xy-0214xy ICAD(Inhibitor of Caspase-Activated DNase )Caspase 激活的脱氧核糖核酸酶抑制剂（抗原）
xy-0018xy IDE胰岛素降解酶（抗原）
xy-0227xy IGF-1 R 人胰岛素样生长因子-I受体
xy-0406xy IGFBP-5 ( Insulin-like Growth Factor Binding Protein 5、IBP5)胰岛素样生长因子结合蛋白-5(抗原)
xy-0056xy Isulin胰岛素（抗原）
xy-0040xy APC(Activated protein C)APC活化蛋白C抗原
xy-2425xy Apelin脂肪炎症因子Apelin抗原
xy-2430xy Apelin receptor/APJ receptor Apelin receptor抗原
xy-1384xy APG4B/AUTL1APG4B细胞自噬相关抗原
xy-0849xy apoA1 (Apolipoprotein/APOA1 protein )human载脂蛋白A1抗原
xy-1354xy Apo J/Clusterin- Alpha/ Beta(Apolipoprotein J)凝聚素α/β抗原
xy-0047xy Insulin Receptor-Alpha(ISR-Alpha)胰岛素受体-α（抗原）
xy-0260xy Insulin Receptor-Alpha(ISR-Alpha)胰岛素受体-α（抗原）
xy-0290xy Insulin Receptor- Beta(ISR- Beta)胰岛素受体-β（抗原）
xy-0172xy IRS-1胰岛素受体底物-1（抗原）
Cdc42 Activation Assay Kitxy-1253xy AQP3(aquaporin 3)水通道蛋白-3抗原
xy-0173xy IRS-2 胰岛素受体底物-2（抗原）
xy-1506xy AQP1(Aquaporin1)水通道蛋白-1抗原
xy-0186xy IRS-3胰岛素受体底物-3（抗原）
xy-0187xy IRS-4胰岛素受体底物-4（抗原）
xy-0242xy IRS P53 (Insulin receptor substrate P53)(多肽)
xy-2639xy PGE2 peptide前列腺素E2抗原
xy-0342xy Integrin Beta chain (ITG- Beta3/ITGB3)整合素-β3（抗原）
xy-2060xy AQP9(aquaporin Protein-9)水通道蛋白-9抗原 (多肽抗原)
xy-0108xy Leptin瘦素（多肽抗原）
xy-0109xy Leptin receptor(long)瘦素受体（长）（抗原）
xy-0943xy phospho-AR/androgen receptor( p-Ser580)peptide雄激素受体（多肽）
xy-0022xy MAPK-1/2丝裂原活化蛋白激酶（抗原）
xy-0223xy MAPKK2丝裂原活化蛋白激酶激酶
Cdc42 Activation Assay Kitxy-0101xy M2-PK ( pyruvate Kinase M2)丙酮酸激酶-M2（抗原）
xy-0102xy M2-PK ( pyruvate Kinase M2 )丙酮酸激酶-M2（抗原）