- 询价
- ATCC
- 美国/中国
- xy-T331M
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Rhodamine tubulin, frozen
- 库存：
1
- 供应商：
上海信裕
- Rhodamine tubulin, frozen详细信息：
- Product Uses Include
- Monitoring microtubule dynamcs in living cells by microinjection (TL331M & T331M)
- Formation of fluorescent microtubules (TL331M, T331M & T331)
- Microscopy studies of MAP and microtubule associated motor activities (TL331M, T331M & T331)
- Nanotechnology (TL331M, T331M & T331)
Rhodamine tubulin, frozenMaterial
Bovine brain tubulin (>99% pure, see Cat. # TL238) has been modified to contain covalently linked rhodamine at random surface lysines. An activated ester of the fluorochrome [(5-(and 6)-carboxytetramethylrhodamine succinimidyl ester] was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations (dye extinction coefficient when protein bound is 70,000M-1cm-1). Final labeling stoichiometry is 1-2 dyes per tubulin heterodimer. Rhodamine labeled tubulin can be detected using a filter set of 535 nm excitation and 585 emission.
Rhodamine tubulin, frozen
xy-0374R-xyE-xyY5 Rabbit Anti-horse IgM/PE-CY5PE-CY5标记的兔抗马IgM
xy-0308R-xyE-xyy3 Rabbit Anti-horse IgG/PE-Cy3PE-Cy3标记的兔抗马IgG
xy-0345G-xyy5 Goat Anti-human IgM/Cy5Cy5标记的羊抗人IgM
xy-0345G-FITxy Goat Anti-human IgM/FITCFITC标记的羊抗人IgM
xy-0297G-FITxy Goat Anti-human IgG/FITCFITC标记的羊抗人IgG
xy-0297G-Golxy Goat Anti-human IgG/Gold胶体金标记的羊抗人IgG
xy-0297R-FITxy Rabbit Anti-human IgG/FITCFITC标记的兔抗人IgG
xy-0374R-HRxy Rabbit Anti-horse IgM/HRP辣根过氧化物酶标记的兔抗马IgM
xy-0297G-AF488 Goat Anti-human IgG/Alexa Fluor 488Alexa Fluor 488标记的羊抗人IgG
xy-0297M-AF647 Mouse Anti-human IgG/Alexa Fluor 647Alexa Fluor 647标记的小鼠抗人IgG
xym-0297M-xyE Mouse Anti-human IgG(3D3)/PEPE标记的鼠抗人IgG单克隆抗体
xy-0360R-HRxy Rabbit Anti-human sIgA/HRP辣根过氧化物酶标记兔抗人IgA
xym-0297M-Bio Mouse Anti-human IgG(3D3)/Bio生物素标记的鼠抗人IgG单克隆抗体
xy-0297R-Bio Rabbit Anti-human IgG/Bio生物素标记的兔抗人IgG
xy-0297M-Bio Mouse Anti-human IgG/Bio生物素标记的小鼠抗人IgG
xy-0360R-Bio Rabbit Anti-human sIgA/Bio生物素标记的兔抗人分泌型IgA
xy-0345G-Bio Goat Anti-human IgM/Bio生物素标记的羊抗人IgM
xy-0297xy-AF488 Donkey Anti-human IgG/Alexa Fluor 488Alexa Fluor 488标记的驴抗人IgG
xy-0297R-xyE-xyY5 Rabbit Anti-human IgG/PE-CY5PE-CY5标记的兔抗人IgG
xy-0297M-xyE Mouse Anti-human IgG/PEPE标记的小鼠抗人IgG
xy-0297R-AF647 Rabbit Anti-human IgG/Alexa Fluor 647Alexa Fluor 647标记的兔抗人IgG
xy-0403R-Bio Rabbit anti-MG IgG/Bio生物素标记的兔抗长爪沙鼠IgG
xy-0377R-xyE-xyy5.5 Rabbit Anti-mouse IgG-Fc/PE-Cy5.5PE-Cy5.5标记的兔抗小鼠IgG-Fc
xy-0377R-xyE-xyy7 Rabbit Anti-mouse IgG-Fc/PE-Cy7PE-Cy7标记的兔抗小鼠IgG-Fc
xy-0377R-xyE-xyy3 Rabbit Anti-mouse IgG-Fc/PE-Cy3PE-Cy3标记的兔抗小鼠IgG-Fc
xy-0377R-xyE-xyY5 Rabbit Anti-mouse IgG-Fc/PE-CY5PE-CY5标记的兔抗小鼠IgG-Fc
xy-0330R-Bio Rabbit anti-mouse Kappa light chain/Bio生物素标记的兔抗小鼠k链
xy-0336R-AF647 Rabbit Anti-MK IgM/Alexa Fluor 647Alexa Fluor 647标记的兔抗猴IgM
xy-0330R-xyE Rabbit anti-mouse Kappa light chain/PEPE标记的兔抗小鼠k链
xy-0330R-xyE-xyY5 Rabbit anti-mouse Kappa light chain/PE-CY5PE-CY5标记的兔抗小鼠k链
xy-0330R-AF488 Rabbit anti-mouse Kappa light chain/Alexa Fluor 488Alexa Fluor 488标记的兔抗小鼠k链
xy-0330R-AF647 Rabbit anti-mouse Kappa light chain/Alexa Fluor 647Alexa Fluor 647标记的兔抗小鼠k链
Rhodamine tubulin, frozenxy-0330R-xyE-xyy5.5 Rabbit anti-mouse Kappa light chain/PE-Cy5.5PE-Cy5.5标记的兔抗小鼠k链
xy-0377R-FITxy Rabbit Anti-mouse IgG-Fc/FITCFITC标记的兔抗小鼠IgG-Fc
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文献和实验Large Scale Tubulin Preparation
when handed to you at the slaughterhouse) -- yields decline significantly if the brains have been stored for a while after removal. Frozen brains do not work for preparing tubulin. The best preps have been done with freshly removed brains transported in an ice
Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
Materials 1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.Stored at -80℃ 2.GTP,100 mM stock,titrate to pH ~7.Need 2 ml. 3.Waterbath,set at 37℃ 4.SS34 rotor,5 ml and 15 ml tubes and adaptors.Bring the rotor,adaptors,and tubes
Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe
The fission yeast Schizosaccharomyces pombe is an attractive source of tubulin for biochemical experiments as it contains few tubulin isoforms and is amenable to genetic manipulation. We describe the preparation of milligram quantities
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