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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Integrin β1 (Activating) Monoclonal Antibody
- 库存：
1
- 供应商：
上海信裕
- Integrin β1 (Activating) Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description 
Monoclonal Antibody against a Calcium Dependent Activation Epitope on Integrin β1 Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone P4G11 Form Ascites Host Mouse Species Reactivity Integrin β1 (Activating) Monoclonal Antibody Human IsoType IgG1 [Ab] 2-3 mg/mL Specificity The P4G11 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs). P4G11, reacts with calcium-dependent epitopes on the integrin β1 subunit. When bound to β1, P4G11, activates β1 dependent binding to extra cellular matrix substrates. Uses This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:1,000
- IF: 1:500
- IP: 1:150
Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Wayner EA, Hoffstrom BG. Monoclonal antibodies to integrin receptors. Meths. Enzymol. In: Integrins, edited by David Cheresh, 426:117-154, 2007. [General Use]
Wayner EA, Gil SG, Murphy GF, Wilke MS, Carter WG. Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes. J Cell Biol 121(5):1141-1152, 1993. [IP]Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Integrin β1 (Activating) Monoclonal Antibody
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文献和实验spreading, we developed a method engaging integrin signaling by use of an immobilized anti-integrin monoclonal antibody (mab) directed against the fibronectin (FN) receptor integrin α5β1. ECV 304 cells were plated onto FN or immobilized mab JBS5 (anti
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Peptide Synthesis in the Study of Collagen-Platelet Interactions
represents a very accessible example of this principle in operation, utilizing direct interaction with integrin α2 β1 and glycoprotein (GP) VI, and indirect interaction with GPIb via von Willebrand factor. Binding sites within collagen and their complementary
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