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- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Tubulin polymerization assay
- 库存：
1
- 供应商：
上海信裕
- Tubulin polymerization assay详细信息：
- Product Uses Include
- Cost effective high throughput screen for anti-cancer compounds.
- Basic research to measure compound IC50s and specificity for tubulin.
- Screening proteins for effects on tubulin polymerization activity.
- Teaching aid for undergraduate/graduate class in pharmacology.
Tubulin polymerization assayIntroduction
This assay is an economical one step procedure for determining the effects of drugs or proteins on tubulin polymerization. It is an adaptation of an assay originally described by Bonne, D. et al. (1). Polymerization is followed by fluorescence enhancement due to the incorporation of a fluorescent reporter into microtubules as polymerization occurs. The standard assay uses neuronal tubulin (Cat. # TL238), which generates a polymerization curve representing the three phases of microtubule formation; namely nucleation, growth and steady state equilibrium. Other tubulins, such as HeLa cell tubulin (Cat. # H001) can also be used in this assay. The low volume per assay of 50 µl and the low tubulin concentrations, 2 mg/ml final concentration (100 µg per assay), makes this an ideal choice for studying the more expensive cancer cell tubulin reagents and for high throughput applications.
Tubulin polymerization assay
xy-0777R-xyE-xyy3 Rabbit Anti-Mink IgG/PE-Cy3PE-Cy3标记的兔抗水貂IgG
xy-0777R-xyE-xyY5 Rabbit Anti-Mink IgG/PE-CY5PE-CY5标记的兔抗水貂IgG
xy-0777R-Axyxy Rabbit Anti-Mink IgG/APCAPC标记的兔抗水貂IgG
xy-0777R-AF350 Rabbit Anti-Mink IgG/Alexa Fluor 350Alexa Fluor 350标记的兔抗水貂IgG
xy-0777R-AF488 Rabbit Anti-Mink IgG/Alexa Fluor 488Alexa Fluor 488标记的兔抗水貂IgG
xy-0777R-AF555 Rabbit Anti-Mink IgG/Alexa Fluor 555Alexa Fluor 555标记的兔抗水貂IgG
xy-0777R-AF647 Rabbit Anti-Mink IgG/Alexa Fluor 647Alexa Fluor 647标记的兔抗水貂IgG
xy-0777R-xyE-xyy5.5 Rabbit Anti-Mink IgG/PE-Cy5.5PE-Cy5.5标记的兔抗水貂IgG
xy-0777R-xyE-xyy7 Rabbit Anti-Mink IgG/PE-Cy7PE-Cy7标记的兔抗水貂IgG
xy-0777R-Axy Rabbit Anti-Mink IgG/AP碱性磷酸酶（AP）标记的兔抗水貂IgG
xy-0777R-FITxy Rabbit Anti-Mink IgG/FITCFITC标记的兔抗水貂IgG
xy-0777R-RBITxy Rabbit Anti-Mink IgG/RBITC罗丹明标记的兔抗水貂IgG
xy-0777R-Golxy Rabbit Anti-Mink IgG/Gold胶体金标记的兔抗水貂IgG
xy-0297xy-RBITxy Donkey Anti-human IgG/RBITC罗丹明标记的驴抗人IgG
xy-0297xy-Golxy Donkey Anti-human IgG/Gold胶体金标记的驴抗人IgG
xy-0297xy-FITxy Donkey Anti-human IgG/FITCFITC标记的驴抗人IgG
xy-0297xy-Axy Donkey Anti-human IgG/AP碱性磷酸酶（AP）标记的驴抗人IgG
xy-0297xy-HRxy Donkey Anti-human IgG/HRP辣根过氧化物酶标记的驴抗人IgG
xy-0297xy-Bio Donkey Anti-human IgG/Bio生物素标记的驴抗人IgG
xy-0297xy-AF555 Donkey Anti-human IgG/Alexa Fluor 555Alexa Fluor 555标记的驴抗人IgG
xy-0297xy-AF647 Donkey Anti-human IgG/Alexa Fluor 647Alexa Fluor 647标记的驴抗人IgG
xy-0297xy-xyE-xyy5.5 Donkey Anti-human IgG/PE-Cy5.5PE-Cy5.5标记的驴抗人IgG
xy-0297xy-xyE-xyy7 Donkey Anti-human IgG/PE-Cy7PE-Cy7标记的驴抗人IgG
xy-0297xy-xyy3 Donkey Anti-human IgG/Cy3Cy3标记的驴抗人IgG
xy-0297xy-xyy5 Donkey Anti-human IgG/Cy5Cy5标记的驴抗人IgG
xy-0297xy-xyy5.5 Donkey Anti-human IgG/Cy5.5Cy5.5标记的驴抗人IgG
xy-0297xy-xyy7 Donkey Anti-human IgG/Cy7Cy7标记的驴抗人IgG
xy-0297xy-xyE Donkey Anti-human IgG/PEPE标记的驴抗人IgG
xy-0297xy-xyE-xyy3 Donkey Anti-human IgG/PE-Cy3PE-Cy3标记的驴抗人IgG
xy-0297xy-xyE-xyY5 Donkey Anti-human IgG/PE-CY5PE-CY5标记的驴抗人IgG
xy-0297xy-Axyxy Donkey Anti-human IgG/APCAPC标记的驴抗人IgG
xy-0297xy-AF350 Donkey Anti-human IgG/Alexa Fluor 350Alexa Fluor 350标记的驴抗人IgG
xy-0295xy-xyE-xyy5.5 Donkey Anti-rabbit IgG/PE-Cy5.5PE-Cy5.5标记的驴抗兔IgG
Tubulin polymerization assayxy-0295xy-xyE-xyy7 Donkey Anti-rabbit IgG/PE-Cy7PE-Cy7标记的驴抗兔IgG
xy-0295xy-xyy3 Donkey Anti-rabbit IgG/Cy3Cy3标记的驴抗兔IgG
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文献和实验Tubulin Polymerization with GTP/GMPCPP/Taxol
GMPCPP and add a small volume of the seeds (~1/20-1/50 vol) after the polymerization mix has been at 37¡C for 1'. Axonemes or centrosomes can also be used as nucleating structures. If the purpose is labeling or recycling the tubulin, polymerization
In Vivo Actin Polymerization ( cAMP)
Chemicals and equipments Actin Buffer ( 20 mM KPO4, 10 mM PIPES, 5 mM EGTA, 2 mM MgCl2, pH 6.8), Assay buffer (Actin Buffer with 3.7 formaldehyde, 0.1 triton, 200X TRITC- Phaloidin from 50 mM stock.), DB, Prepare Cells
on ice. Use the thawed tubulin for polymerization preferably within 30' of thawing. When quantitative analysis of tubulin polymerization is necessary, careful and consistent handling of tubulin aliquots is essential for reproducible results.
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