- 询价
- ATCC
- 美国/中国
- xy-BK006
- 2025年07月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Tubulin polymerization assay kit
- 库存：
1
- 供应商：
上海信裕
- Tubulin polymerization assay kit详细信息：
- Product Uses Include
- Screening compounds for effects on tubulin polymerization activity.
- Screening proteins for effects on tubulin polymerization activity.
- Determining that tubulin alone (not MAPs) is the target for compounds that affect microtubule polymerization.
- Teaching aid for undergraduate/graduate class in pharmacology.
Tubulin polymerization assay kitIntroduction
This assay is based on an adaptation of the original method of Shelanski et al. and Lee et al. (1,2), which demonstrated that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer. The resulting polymerization curve is representative of the three phases of microtubule polymerization, namely nucleation, growth and steady state equilibrium. See the About Tubulin page for more information. The assay is optimized for a 96-well format for low CVs and efficient sample handling.This kit contains the bovine neuronal tubulin of the highest available purity (>99% pure, Cat. # TL238). The same type of assay is also available with HTS tubulin (Cat. # BK004/CDS01) and can serve as an economical, but slightly less sensitive alternative to BK006/CDS03. HTS tubulin (Cat. # HTS02) is >97% pure. Cytoskeleton, Inc. also provides a fluorescence based tubulin polymerization assay in miniaturized format (Cat. # BK011) making it ideal for high throughput screening.If you are interested in using either of these tubulin polymerization assays in a high throughput setting, please contact our technical service department for advice and bulk quotes.
Tubulin polymerization assay kit
xy-0295xy-xyy5.5 Donkey Anti-rabbit IgG/Cy5.5Cy5.5标记的驴抗兔IgG
xy-0295xy-xyy7 Donkey Anti-rabbit IgG/Cy7Cy7标记的驴抗兔IgG
xy-0295xy-xyE Donkey Anti-rabbit IgG/PEPE标记的驴抗兔IgG
xy-0295xy-xyE-xyy3 Donkey Anti-rabbit IgG/PE-Cy3PE-Cy3标记的驴抗兔IgG
xy-0295xy-xyE-xyY5 Donkey Anti-rabbit IgG/PE-CY5PE-CY5标记的驴抗兔IgG
xy-0295xy-Axyxy Donkey Anti-rabbit IgG/APCAPC标记的驴抗兔IgG
xy-0295xy-AF350 Donkey Anti-rabbit IgG/Alexa Fluor 350Alexa Fluor 350标记的驴抗兔IgG
xy-0295xy-RBITxy Donkey Anti-rabbit IgG/RBITC罗丹明标记的驴抗兔IgG
xy-0295xy-Golxy Donkey Anti-rabbit IgG/Gold胶体金标记的驴抗兔IgG
xy-0294xy-Golxy Donkey Anti-Goat IgG/Gold胶体金标记的驴抗羊IgG
xy-0294xy-Axy Donkey Anti-Goat IgG/AP碱性磷酸酶（AP）标记的驴抗羊IgG
xy-0294xy-RBITxy Donkey Anti-Goat IgG/RBITC罗丹明标记的驴抗羊IgG
xy-0294xy-AF488 Donkey Anti-Goat IgG/Alexa Fluor 488Alexa Fluor 488标记的驴抗羊IgG
xy-0294xy-xyy5.5 Donkey Anti-Goat IgG/Cy5.5Cy5.5标记的驴抗羊IgG
xy-0294xy-xyy7 Donkey Anti-Goat IgG/Cy7Cy7标记的驴抗羊IgG
xy-0294xy-xyE Donkey Anti-Goat IgG/PEPE标记的驴抗羊IgG
xy-0294xy-xyE-xyy3 Donkey Anti-Goat IgG/PE-Cy3PE-Cy3标记的驴抗羊IgG
xy-0294xy-xyE-xyY5 Donkey Anti-Goat IgG/PE-CY5PE-CY5标记的驴抗羊IgG
xy-0294xy-Axyxy Donkey Anti-Goat IgG/APCAPC标记的驴抗羊IgG
xy-0294xy-xyy3 Donkey Anti-Goat IgG/Cy3Cy3标记的驴抗羊IgG
xy-0294xy-AF555 Donkey Anti-Goat IgG/Alexa Fluor 555Alexa Fluor 555标记的驴抗羊IgG
xy-0294xy-xyE-xyy7 Donkey Anti-Goat IgG/PE-Cy7PE-Cy7标记的驴抗羊IgG
xy-0358xy-Golxy Donkey Anti-Guinea pig IgG/Gold胶体金标记的驴抗豚鼠IgG
xy-0358xy-xyE-xyy5.5 Donkey Anti-Guinea pig IgG/PE-Cy5.5PE-Cy5.5标记的驴抗豚鼠IgG
xy-0358xy-xyy3 Donkey Anti-Guinea pig IgG/Cy3Cy3标记的驴抗豚鼠IgG
xy-0358xy-AF488 Donkey Anti-Guinea pig IgG/Alexa Fluor 488Alexa Fluor 488标记的驴抗豚鼠IgG
xy-0358xy-xyy5.5 Donkey Anti-Guinea pig IgG/Cy5.5Cy5.5标记的驴抗豚鼠IgG
xy-0358xy-xyE-xyY5 Donkey Anti-Guinea pig IgG/PE-CY5PE-CY5标记的驴抗豚鼠IgG
xy-0358xy-Axyxy Donkey Anti-Guinea pig IgG/APCAPC标记的驴抗豚鼠IgG
xy-0358xy-Axy Donkey Anti-Guinea pig IgG/AP碱性磷酸酶（AP）标记的驴抗豚鼠IgG
xy-0358xy-FITxy Donkey Anti-Guinea pig IgG/FITCFITC标记的驴抗豚鼠IgG
xy-0358xy-RBITxy Donkey Anti-Guinea pig IgG/RBITC罗丹明标记的驴抗豚鼠IgG
Tubulin polymerization assay kitxym-0297M-RBITxy Mouse Anti-human IgG(3D3)/RBITC罗丹明标记的鼠抗人IgG单克隆抗体
xym-0297M-Golxy Mouse Anti-human IgG(3D3)/Gold胶体金标记的鼠抗人IgG单克隆抗体
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文献和实验Tubulin Polymerization with GTP/GMPCPP/Taxol
GMPCPP and add a small volume of the seeds (~1/20-1/50 vol) after the polymerization mix has been at 37¡C for 1'. Axonemes or centrosomes can also be used as nucleating structures. If the purpose is labeling or recycling the tubulin, polymerization
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
In Vivo Actin Polymerization ( cAMP)
Chemicals and equipments Actin Buffer ( 20 mM KPO4, 10 mM PIPES, 5 mM EGTA, 2 mM MgCl2, pH 6.8), Assay buffer (Actin Buffer with 3.7 formaldehyde, 0.1 triton, 200X TRITC- Phaloidin from 50 mM stock.), DB, Prepare Cells
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