PI3-Kinase Activity ELISA Assa

The K-1000 kit will be discontinued on 8/2011. For a substitute product, please see the K-1000s - PI3-Kinase Activity ELISA: Pico. The K-1000s is over 50x more sensitive for PI(3,4,5)P3 the enzymatic product of PI3K, uses less enzyme and contains an optimized buffer for high PI3K activity. Please see the>PI3-K Assay Comparison Chart for more information.

PI3-Kinase Activity ELISA AssayThe assay is a competitive ELISA where the signal is inversely proportional to the amount of PI(3,4,5)P3 produced. After the PI3-K reactions are complete, reaction products are mixed and incubated with a PI(3,4,5)P3 detector protein, then added to the PI(3,4,5)P3-coated microplate for competitive binding. A peroxidase-linked secondary detection reagent and colorimetric detection is used to detect PI(3,4,5)P3 detector protein binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(3,4,5)P3 produced by PI3-K activity.

Class I Phosphoinositide 3-kinases (PI3-K), are ubiquitously expressed lipid kinases which phosphorylate PI(4,5)P2 at the 3-hydroxyl of the inositol ring producing PtdIns(3,4,5)P3. PtdIns(3,4,5)P3 has shown to be an important lipid second messenger with key roles in fundamental cellular responses such as proliferation and survival. Due to these interesting biological roles, PI3-K and PtdIns(3,4,5)P3 have become attractive targets for research and drug development in many diseases including inflammation and cancer. Echelon has multiple formats available for the determination of class I PI3-K activity.

PI3-Kinase Activity ELISA AssayBulk Discounts

• Bulk discounts are available, please email echelon@echelon-inc.com for details

Benefits

• Quick, stable and sensitive

Storage

• 2-8 °C, Do not freeze thaw multiple times

Applications

• in vitro determination of PI3Kactivity in cancer tissues and cell lines- Compound screening for drug discovery
• PI3-Kinase Activity ELISA Assay
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