PI3-Kinase Activity ELISA

Description: PI3-Kinase Activity Pico Assay is a competitive ELISA for measuring PI3-Kinase activity in a 96-well format. The assay detects low levels of PI(3,4,5)P₃ produced in in vitro PI3-Kinase reactions.

PI3-Kinase Activity ELISAAfter the PI3-K reactions are complete, reaction products are mixed and incubated with a PI(3,4,5)P₃ detector protein, then added to the PI(3,4,5)P₃-coated microplate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect PI(3,4,5)P₃ detector protein binding to the plate. The colorimetric signal is inversely proportional to the amount of PI(3,4,5)P₃ produced by PI3-K activity.

Class I Phosphoinositide 3-kinases (PI3-K), are ubiquitously expressed lipid kinases which phosphorylate PI(4,5)P₂ at the 3-hydroxyl of the inositol ring producing PtdIns(3,4,5)P₃. PtdIns(3,4,5)P₃ has shown to be an important lipid second messenger with key roles in fundamental cellular responses such as proliferation and survival. Due to these interesting biological roles, PI3-K and PtdIns(3,4,5)P₃ have become attractive targets for research and drug development in many diseases including inflammation and cancer. Echelon has multiple formats available for the determination of class I PI3-K activity.

PI3-Kinase Activity ELISABulk Discounts

• Bulk discounts are available, please email wdwu@qcbio.com for details

Storage

• 2-8 °C

Applications

• In vitro determination of PI3K activity in cancer tissues and cell lines.

• Compound screening for drug discovery
• PI3-Kinase Activity ELISA
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