Ubiquitin (P4G7) Monoclonal Antibody Extensive (Yeast to Human)
IsoType
IgG1
[Ab]
3-5 mg/mL
Specificity
Antibody P4D1 was raised against denatured bovine ubiquitin and recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins. MAb P4D1 is equivalent to MAb P4G7. The ubiquitin protein is extremely well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and ubiquitin-substrate conjugates by immunoblotting. Because the antibody recognizes a denatured epitope, it is known not to work in immunoprecipitation or immunostaining applications.
Uses
This antibody is effective in immunoblotting and ELISA.
Suggested Working Dilution
The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
References
Kahana A, Gottschling DE. DOT4 Links Silencing and Cell Growth in Saccharomyces cerevisiae. Mol. Cell. Biol. 19(10):6608-6620, 1999.
Hochstrasser M. Ubiquitin-dependent protein degradation. Annu. Rev. Genet. 30:405-439, 1996.
Warranty/Conditions
Covance products may not be resold or modified for resale without prior written approval.