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- MEK 1/2 [Ser 218/222] Polyclonal Antibody, Phospho-Specific保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description ![MEK 1/2 [Ser 218/222] Polyclonal Antibody, Phospho-Specific - SIGNAL TRANSDUCTION](https://store.crpinc.com/images/spacer.gif)
Phospho-Specific polyclonal antibody to MEK 1/2 [Ser 218/222] Form Affinity Purified Antibody (Stored in 10 mM Hepes pH 7.5, 150 mM NaCl, 100 ug/mL BSA and 50% glycerol) Host MEK 1/2 [Ser 218/222] Polyclonal Antibody, Phospho-Specific Rabbit Species Reactivity Human Specificity This antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphor Ser218/222 of human MEK 1/2. It is specific for Mr 45kD MEK 1/2 phosphorylated at Ser 218 or Ser 222 in serum starved NIH/3T3 cells. This antibody was purified by sequential chromatography on Protein A and phospho and dephospho peptide affinity columns. Uses These antibodies are effective in immunoblotting, immunohistochemistry, immunoprecipitation and ELISA. Suggested Working Dilution MEK 1/2 [Ser 218/222] Polyclonal Antibody, Phospho-Specific Quality control tests are done by Western and Dot blot. The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000*
- Immunohistochemistry 1:1,000
- Immunoprecipitation 1:1,000
- ELISA 1:1,000
Notes *Predicted MW= 45kD Storage Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw. Avoid multiple freeze thaw cycles. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. Rev. Date 1/1/2004 MEK 1/2 [Ser 218/222] Polyclonal Antibody, Phospho-Specific - xy-11225R Phospho-ATRIP (Ser224)磷酸化系统性红斑狼疮相关蛋白TREX1抗体
xy-11241R AP2M1接头相关蛋白复合体AP-2μ链1抗体
xy-11242R Phospho-AP2M1 (Thr156)磷酸化接头相关蛋白复合体AP-2μ链1抗体
xy-4901R Annexin A7膜粘连蛋白7抗体
xy-9823R C3orf153号染色体开放阅读框15抗体
xy-6336R APOB48R载脂蛋白B受体抗体
xy-6332R ABCA2三磷酸腺苷结合盒转运蛋白2抗体
xy-6333R ApoB 载脂蛋白B抗体
xy-6335R ALOX15B花生四烯酸15脂氧合酶2抗体
xy-6338R APOC4载脂蛋白C4抗体
xy-6337R ARH低密度脂蛋白受体衔接蛋白抗体
xy-7550R AUP1原始广泛存在蛋白1抗体
xy-9398R ANKRD37锚蛋白重复结构域蛋白37抗体
xy-7588R A4GALTα1,4-半乳糖基转移酶1
xy-7642R AGTRAP血管紧张素Ⅱ受体相关蛋白抗体
xy-7669R AX2R膜粘连蛋白2受体抗体
xy-0203R ACEI血管紧张素1转换酶抑制剂抗体
xy-1229R AATF拮抗凋亡转录因子抗体
xy-0094R AACT-Alpha1α-1抗胰糜蛋白酶抗体
xy-0096R alpha 1 Antitrypsinα-1抗胰蛋白酶抗体
xy-1224R ABCB6ATP结合蛋白家族6抗体
xy-1231R ABCG1三磷酸腺苷结合盒亚家族G1抗体
xy-0611R Adiponectin receptor 2脂联素受体2抗体
xy-9741R ANGEL1ANGEL1蛋白抗体
xy-9742R ANGEL2ANGEL2蛋白抗体
xy-9743R ANKLE2ANKLE2蛋白抗体
xy-9744R ANKMY1睾丸特异性锚样蛋白1抗体
xy-9745R ANKRD13B锚蛋白重复结构域蛋白13B抗体
xy-9746R ANKRD20A1锚蛋白重复结构域蛋白20A1抗体
xy-9747R ANKRD20A3锚蛋白重复结构域蛋白20A3抗体
xy-9748R ANKRD22锚蛋白重复结构域蛋白22抗体
xy-9751R ANKRD50锚蛋白重复结构域蛋白50抗体
xy-9753R ATP6V1G2氢离子转运ATP合成酶6G抗体
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
) which is probably the best choice, in particular for phospho-specific Abs. Good luck! 真爱满行囊 非常感谢楼上的答复，现在明白了。哎，这个抗体现在就不知道怎么能做出结果了。。。。。再试多几次吧。。。 真爱满行囊 非常感谢，因为进实验室没有多久，还是感觉到现在看的文献太少，对这些问题理解太浅，在看文献的过程中，我还是没有办法理解一个问题，那就是gsk
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
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