- 询价
- ATCC
- xy-03703126001
- 美国/中国
- 2025年07月05日
14 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
上海信裕
详细信息：
Annexin-V-Alexa 568 Roche
Application
The analysis of phosphatidylserine on the outer leaflet of apoptotic cell membranes is performed using Annexin-V-Alexa 568 in combination with a DNA stain (e.g., BOBO-1) to differentiate apoptotic cells from necrotic cells. Apoptotic and necrotic cells can also be differentiated by cell surface markers.
Benefits
Detection flexibility: Analysis of results with flow cytometry and/or fluorescence microscopy
Alexa is a red dye, which can be used for double or triple staining with other fluorescent markers (e.g., fluorescein-labeled surface markers)
Accuracy: Accurately distinguish apoptotic cells from necrotic cells
Stability of results: High photostability (long observation possible)
Rapid and simple procedure
Product Description
Annexin-V-Alexa 568 RocheAlexa 568 is a fluorescent label. The Annexin-V-Alexa 568 conjugate can be easily analyzed on a 488-nm laser Fluorescent Analytical Cell Sorter (FACS) machine, or using a fluorescence microscope. Excitation is in the range of 488 - 596 nm with emission above 600 nm.
Additional secondary labeling is also possible. For example, membrane surfaces can be stained with fluorescein-labeled monoclonal antibodies for further cellular characterization.
Background Information
In the early stages of apoptosis, changes occur on the cell surface. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane to the outer layer, thereby exposing PS at the external surface of the cell. Fadok et al. showed that macrophages specifically recognize PS exposed on the surface of lymphocytes during apoptosis. This recognition and subsequent phagocytosis of apoptotic cells and bodies protects organisms from exposure to cellular compounds that lead to inflammation and usually accompany necrosis.
Annexin-V is a Ca2+-dependent, phospholipid-binding protein with a high affinity for PS. This protein can therefore be used as a sensitive probe for PS exposure on the outer leaflet of the cell membrane, and is suited to detect apoptotic cells. Since necrotic cells also expose PS as a result of lost membrane integrity, apoptotic cells must be differentiated from these necrotic cells. The simultaneous application of a DNA stain (used for dye-exclusion tests [e.g., BOBO-1]) allows the discrimination of necrotic cells from the Annexin-V positively stained cell cluster.
Contents
500 µl, ready-to-use solution, for 250 tests
Annexin-V-Alexa 568 Roche
xy1235 MN-dsc 小鼠背脊髓神经元 Mouse Neurons-dorsal spinal cord 1 x 10^6 cells/vial ATCC
xy1236 MN-sc 小鼠脊髓神经元 Mouse Neurons-spinal cord 1 x 10^6 cells/vial ATCC
xy1237 MOPC 小鼠少突胶质前体细胞 Mouse Oligodendrocyte Precursor Cells 5 x 10^5 cells/vial ATCC
xy1238 MSC 小鼠雪旺细胞 Mouse Schwann Cells 5 x 10^5 cells/vial ATCC
xy1239 MPNF 小鼠神经束膜细胞 Mouse Perineural Fibroblasts 5 x 10^5 cells/vial ATCC
xy1240 MA 小鼠星形胶质细胞 Mouse Astrocytes 5 x 10^5 cells/vial ATCC
xy1241 MA-c 小鼠小脑星形胶质细胞 Mouse Astrocytes-cerebellar 5 x 10^5 cells/vial ATCC
xy1242 MA-h 小鼠海马趾星形胶质细胞 Mouse Astrocytes-hippocampal 5 x 10^5 cells/vial ATCC
xy1243 MA-sc 小鼠脊髓星形胶质细胞 Mouse Astrocytes-spinal cord 5 x 10^5 cells/vial ATCC
xy1244 MM 小鼠小胶质细胞 Mouse Microglia 5 x 10^5 cells/vial ATCC
xy1245 MMa-bm 小鼠巨噬细胞 Mouse Macrophage 1 x 10^6 cells/vial ATCC
xy1246 MLF 小鼠淋巴成纤维细胞 Mouse Lymphatic Fibroblasts 5 x 10^5 cells/vial ATCC
xy1247 MRTEpiC 小鼠肾小管上皮细胞 Mouse Renal Tubular Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1248 MRMC 小鼠肾系膜细胞 Mouse Renal Mesangial Cells 5 x 10^5 cells/vial ATCC
xy1249 MCM 小鼠心肌细胞 Mouse Cardiac Myocytes 1 x 10^6 cells/vial ATCC
xy1250 MCF 小鼠心脏成纤维细胞 Mouse Cardiac Fibroblasts 5 x 10^5 cells/vial ATCC
xy1251 MMSC-bm 小鼠骨髓间充质干细胞 Mouse Mesenchymal Stem Cells-bone marrow 5 x 10^5 cells/vial ATCC
xy1252 MEF 小鼠胚胎成纤维细胞 Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial ATCC
xy1253 MEF-mt 小鼠胚胎成纤维细胞--霉素处理 Mouse Embryonic Fibroblasts-mitomicine treated 5 x 10^5 cells/vial ATCC
xy1254 ATCC
xy1255 CD57BL/6小鼠细胞 ATCC
xy1256 MMC 小鼠脑膜细胞 Mouse Meningeal Cells 5 x 10^5 cells/vial ATCC
xy1257 MN-r 小鼠脑脊神经元 Mouse Neurons-raphe 1 x 10^6 cells/vial ATCC
xy1258 MN-c 小鼠皮质神经元 Mouse Neurons-cortical 1 x 10^6 cells/vial ATCC
xy1259 MGC 小鼠颗粒细胞 Mouse Granule Cells 1 x 10^6 cells/vial ATCC
xy1260 MN-h 小鼠海马趾神经元 Mouse Neurons-hippocampal 1 x 10^6 cells/vial ATCC
xy1261 MN-vsc 小鼠腹脊髓神经元 Mouse Neurons-ventral spinal cord 1 x 10^6 cells/vial ATCC
xy1262 MN-dsc] 小鼠背脊髓神经元 Mouse Neurons-dorsal spinal cord 1 x 10^6 cells/vial ATCC
xy1263 MN-vsc 小鼠腹脊髓神经元 Mouse Neurons-ventral spinal cord 1 x 10^6 cells/vial ATCC
xy1264 MSC 小鼠雪旺细胞 Mouse Schwann Cells 5 x 10^5 cells/vial ATCC
xy1265 MA 小鼠星形胶质细胞 Mouse Astrocytes 5 x 10^5 cells/vial ATCC
Annexin-V-Alexa 568 Rochexy1266 MA-c 小鼠小脑星形胶质细胞 Mouse Astrocytes-cerebellar 5 x 10^5 cells/vial ATCC
xy1267 MA-h 小鼠海马趾星形胶质细胞 Mouse Astrocytes-hippocampal 5 x 10^5 cells/vial ATCC
Annexin-V-Alexa 568 Roche
Application
The analysis of phosphatidylserine on the outer leaflet of apoptotic cell membranes is performed using Annexin-V-Alexa 568 in combination with a DNA stain (e.g., BOBO-1) to differentiate apoptotic cells from necrotic cells. Apoptotic and necrotic cells can also be differentiated by cell surface markers.
Benefits
Detection flexibility: Analysis of results with flow cytometry and/or fluorescence microscopy
Alexa is a red dye, which can be used for double or triple staining with other fluorescent markers (e.g., fluorescein-labeled surface markers)
Accuracy: Accurately distinguish apoptotic cells from necrotic cells
Stability of results: High photostability (long observation possible)
Rapid and simple procedure
Product Description
Annexin-V-Alexa 568 RocheAlexa 568 is a fluorescent label. The Annexin-V-Alexa 568 conjugate can be easily analyzed on a 488-nm laser Fluorescent Analytical Cell Sorter (FACS) machine, or using a fluorescence microscope. Excitation is in the range of 488 - 596 nm with emission above 600 nm.
Additional secondary labeling is also possible. For example, membrane surfaces can be stained with fluorescein-labeled monoclonal antibodies for further cellular characterization.
Background Information
In the early stages of apoptosis, changes occur on the cell surface. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane to the outer layer, thereby exposing PS at the external surface of the cell. Fadok et al. showed that macrophages specifically recognize PS exposed on the surface of lymphocytes during apoptosis. This recognition and subsequent phagocytosis of apoptotic cells and bodies protects organisms from exposure to cellular compounds that lead to inflammation and usually accompany necrosis.
Annexin-V is a Ca2+-dependent, phospholipid-binding protein with a high affinity for PS. This protein can therefore be used as a sensitive probe for PS exposure on the outer leaflet of the cell membrane, and is suited to detect apoptotic cells. Since necrotic cells also expose PS as a result of lost membrane integrity, apoptotic cells must be differentiated from these necrotic cells. The simultaneous application of a DNA stain (used for dye-exclusion tests [e.g., BOBO-1]) allows the discrimination of necrotic cells from the Annexin-V positively stained cell cluster.
Contents
500 µl, ready-to-use solution, for 250 tests
Annexin-V-Alexa 568 Roche
xy1235 MN-dsc 小鼠背脊髓神经元 Mouse Neurons-dorsal spinal cord 1 x 10^6 cells/vial ATCC
xy1236 MN-sc 小鼠脊髓神经元 Mouse Neurons-spinal cord 1 x 10^6 cells/vial ATCC
xy1237 MOPC 小鼠少突胶质前体细胞 Mouse Oligodendrocyte Precursor Cells 5 x 10^5 cells/vial ATCC
xy1238 MSC 小鼠雪旺细胞 Mouse Schwann Cells 5 x 10^5 cells/vial ATCC
xy1239 MPNF 小鼠神经束膜细胞 Mouse Perineural Fibroblasts 5 x 10^5 cells/vial ATCC
xy1240 MA 小鼠星形胶质细胞 Mouse Astrocytes 5 x 10^5 cells/vial ATCC
xy1241 MA-c 小鼠小脑星形胶质细胞 Mouse Astrocytes-cerebellar 5 x 10^5 cells/vial ATCC
xy1242 MA-h 小鼠海马趾星形胶质细胞 Mouse Astrocytes-hippocampal 5 x 10^5 cells/vial ATCC
xy1243 MA-sc 小鼠脊髓星形胶质细胞 Mouse Astrocytes-spinal cord 5 x 10^5 cells/vial ATCC
xy1244 MM 小鼠小胶质细胞 Mouse Microglia 5 x 10^5 cells/vial ATCC
xy1245 MMa-bm 小鼠巨噬细胞 Mouse Macrophage 1 x 10^6 cells/vial ATCC
xy1246 MLF 小鼠淋巴成纤维细胞 Mouse Lymphatic Fibroblasts 5 x 10^5 cells/vial ATCC
xy1247 MRTEpiC 小鼠肾小管上皮细胞 Mouse Renal Tubular Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1248 MRMC 小鼠肾系膜细胞 Mouse Renal Mesangial Cells 5 x 10^5 cells/vial ATCC
xy1249 MCM 小鼠心肌细胞 Mouse Cardiac Myocytes 1 x 10^6 cells/vial ATCC
xy1250 MCF 小鼠心脏成纤维细胞 Mouse Cardiac Fibroblasts 5 x 10^5 cells/vial ATCC
xy1251 MMSC-bm 小鼠骨髓间充质干细胞 Mouse Mesenchymal Stem Cells-bone marrow 5 x 10^5 cells/vial ATCC
xy1252 MEF 小鼠胚胎成纤维细胞 Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial ATCC
xy1253 MEF-mt 小鼠胚胎成纤维细胞--霉素处理 Mouse Embryonic Fibroblasts-mitomicine treated 5 x 10^5 cells/vial ATCC
xy1254 ATCC
xy1255 CD57BL/6小鼠细胞 ATCC
xy1256 MMC 小鼠脑膜细胞 Mouse Meningeal Cells 5 x 10^5 cells/vial ATCC
xy1257 MN-r 小鼠脑脊神经元 Mouse Neurons-raphe 1 x 10^6 cells/vial ATCC
xy1258 MN-c 小鼠皮质神经元 Mouse Neurons-cortical 1 x 10^6 cells/vial ATCC
xy1259 MGC 小鼠颗粒细胞 Mouse Granule Cells 1 x 10^6 cells/vial ATCC
xy1260 MN-h 小鼠海马趾神经元 Mouse Neurons-hippocampal 1 x 10^6 cells/vial ATCC
xy1261 MN-vsc 小鼠腹脊髓神经元 Mouse Neurons-ventral spinal cord 1 x 10^6 cells/vial ATCC
xy1262 MN-dsc] 小鼠背脊髓神经元 Mouse Neurons-dorsal spinal cord 1 x 10^6 cells/vial ATCC
xy1263 MN-vsc 小鼠腹脊髓神经元 Mouse Neurons-ventral spinal cord 1 x 10^6 cells/vial ATCC
xy1264 MSC 小鼠雪旺细胞 Mouse Schwann Cells 5 x 10^5 cells/vial ATCC
xy1265 MA 小鼠星形胶质细胞 Mouse Astrocytes 5 x 10^5 cells/vial ATCC
Annexin-V-Alexa 568 Rochexy1266 MA-c 小鼠小脑星形胶质细胞 Mouse Astrocytes-cerebellar 5 x 10^5 cells/vial ATCC
xy1267 MA-h 小鼠海马趾星形胶质细胞 Mouse Astrocytes-hippocampal 5 x 10^5 cells/vial ATCC
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
A fluorescence-based in vitro assay for investigating early endosome dynamics Sina V Barysch1 , 2 , Reinhard Jahn1 & Silvio O Rizzoli2 ABSTRACT Early endosomes receive material from the plasma membrane by fusion
ylcui: 我给您提供了两个试剂盒的说明书，是CLONTECH公司和ROCHE公司的Annexin V联合PI双染流式细胞学方法检测凋亡细胞的试剂盒说明书。已经发到您的信箱。 其中原理、操作讲得很明确，能够满足您的要求。 购买的话，CLONTECH公司产品找基因公司，ROCHE公司产品代理商很多，但是请您记住比价不能够超过1：9.5，否则就贵了。到货时间都是2周。 如果没记错的话，去年我们花了3600元买了2个50样本的CLONTECH公司的Annexin V联合PI双染流式细胞分析试剂盒。适用
并且很低（不超过30%），不知是什么原因，请教大家应该做如何的改动？我们实验室没有条件做Cr51释放，我都是用CFSE-PI染色或PKH26-annexin V 检测靶细胞死亡。另外，MVA感染的DC与T细胞共培养6-7天时，显微镜下观察和流式检测都发现T细胞有大部分死亡，状态很差。共培养两周后死亡率很高，不知是否正常？感染的DC细胞应先刺激成熟，但不知是感染前刺激还是感染后刺激为好，也不知用什么刺激DC成熟。我用的TNF-a，但效果不好。是否一定要用TNF-a、IL-6、IL-1B、PEG
技术资料暂无技术资料 索取技术资料
Annexin-V-Alexa 568 Roche
询价
