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Annexin-V-Biotin Roche详细信息：
Application
The analysis of phosphatidylserine on the outer leaflet of apoptotic cell membranes is performed using biotin-labeled Annexin-V. Additionally, staining with propidium iodide (PI) in FACS analysis or with a cell surface marker can be used for differentiation from necrotic cells. Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate).
Product Description
Detection: Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate).
Sample material: Cell lines and freshly isolated cells
Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and shows high affinity for phosphatidylserine.
Background Information
Annexin-V-Biotin RocheIn the early stages of apoptosis there are changes occurring on the cell surface, specifically translocation of phosphatidylserine (PS) from the interior side of the plasma membrane to the outer leaflet. This secretion of phosphatidylserine is the ideal basis for Annexin-V, which binds to PS with high affinity. Annexin-V is a Ca2+-dependent phospholipid-binding protein with a high affinity for PS. Since necrotic cells also expose PS as a result of lost membrane integrity, apoptotic cells must be differentiated from these necrotic cells. The simultaneous application of a DNA stain, which is used for dye-exclusion testing, allows the discrimination of necrotic cells from the Annexin-V positively stained cell cluster.
Contents
500 µl, ready-to-use solution, for 250 tests
Typical Experiment
Induce apoptosis.
Wash the cells in PBS.
Incubate cells with Annexin-V-Biotin in a Hepes buffer that contains PI or other labeling reagent for cell surfaces.
Analyze samples under a microscope or on a flow cytometer.
Annexin-V-Biotin Roche
xy1168 HCM 人心肌细胞 Human Cardiac Myocytes 5 x 10^5 cells/vial ATCC
xy1169 HCM-a 人心肌细胞--成人 Human Cardiac Myocytes-adult 5 x 10^5 cells/vial ATCC
xy1170 HCF 人心脏成纤维细胞 Human Cardiac Fibroblasts 5 x 10^5 cells/vial ATCC
xy1171 HCF-av 人心脏成纤维细胞--成人心室 Human Cardiac Fibroblasts-adult ventrical 5 x 10^5 cells/vial ATCC
xy1172 HCF-aa 人心脏成纤维细胞--成人心房 Human Cardiac Fibroblasts-adult atrial 5 x 10^5 cells/vial ATCC
xy1173 HCF-a 人心脏成纤维细胞--成人 Human Cardiac Fibroblasts-adult 5 x 10^5 cells/vial ATCC
xy1174 HCF-fa 人心脏成纤维细胞--胎儿心房 Human Cardiac Fibroblasts-fetal atrial 6 x 10^5 cells/vial ATCC
xy1175 HPcF 人心包成纤维细胞 Human Pericardial Fibroblasts 5 x 10^5 cells/vial ATCC
xy1176 ATCC
xy1177 视觉细胞系统 ATCC
xy1178 HCEpiC 人角膜上皮细胞 Human Corneal Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1179 HK 人角膜细胞 Human Keratocytes 5 x 10^5 cells/vial ATCC
xy1180 HREC 人视网膜内皮细胞 Human Retinal Endothelial Cells 5 x 10^5 cells/vial ATCC
xy1181 HRPEpiC 人视网膜色素上皮细胞 Human Retinal Pigment Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1182 HLEpiC 人晶状体上皮细胞 Human Lens Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1183 HIPEpiC 人虹膜色素上皮细胞 Human Iris Pigment Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1184 HConF 人结膜成纤维细胞 Human Conjunctival Fibroblasts 5 x 10^5 cells/vial ATCC
xy1185 HNPCEpiC 人无色素睫装上皮细胞 Human Non-Pigment Ciliary Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1186 HTMC 人小梁网细胞 Human Trabecular Meshwork Cells 5 x 10^5 cells/vial ATCC
xy1187 HOCF 人眼脉络膜成纤维细胞 Human Ocular Choroid Fibroblasts 5 x 10^5 cells/vial ATCC
xy1188 HConEpiC 人结膜上皮细胞 Human Conjunctival Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1189 ATCC
xy1190 胎盘细胞系统 ATCC
xy1191 HVCEC 人绒毛膜毛细血管内皮细胞 Human Villus Capillary Endothelial Cells 5 x 10^5 cells/vial ATCC
xy1192 HAmEpiC 人羊膜上皮细胞 Human Amniotic Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1193 HVT 人绒毛膜滋养层细胞 Human Villous Trophoblasts 1 x 10^6 cells/vial ATCC
xy1194 HVMF 人绒毛间充质成纤维细胞 Human Villous Mesenchymal Fibroblasts 5 x 10^5 cells/vial ATCC
xy1195 脂肪细胞系统 ATCC
xy1196 HAMEC 人脂肪微血管内皮细胞 Human Adipose Microvascular Endothelial Cells 1 x 10^6 cells/vial ATCC
xy1197 HPA-v 人前脂肪细胞--内脏 Human Preadipocytes-visceral 1 x 10^6 cells/vial ATCC
xy1198 HPA-s 人前脂肪细胞--皮下 Human Preadipocytes-subcutaneous 1 x 10^6 cells/vial ATCC
xy1199 ATCC
xy1200 女性生殖细胞系统 ATCC
Application
The analysis of phosphatidylserine on the outer leaflet of apoptotic cell membranes is performed using biotin-labeled Annexin-V. Additionally, staining with propidium iodide (PI) in FACS analysis or with a cell surface marker can be used for differentiation from necrotic cells. Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate).
Product Description
Detection: Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate).
Sample material: Cell lines and freshly isolated cells
Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and shows high affinity for phosphatidylserine.
Background Information
Annexin-V-Biotin RocheIn the early stages of apoptosis there are changes occurring on the cell surface, specifically translocation of phosphatidylserine (PS) from the interior side of the plasma membrane to the outer leaflet. This secretion of phosphatidylserine is the ideal basis for Annexin-V, which binds to PS with high affinity. Annexin-V is a Ca2+-dependent phospholipid-binding protein with a high affinity for PS. Since necrotic cells also expose PS as a result of lost membrane integrity, apoptotic cells must be differentiated from these necrotic cells. The simultaneous application of a DNA stain, which is used for dye-exclusion testing, allows the discrimination of necrotic cells from the Annexin-V positively stained cell cluster.
Contents
500 µl, ready-to-use solution, for 250 tests
Typical Experiment
Induce apoptosis.
Wash the cells in PBS.
Incubate cells with Annexin-V-Biotin in a Hepes buffer that contains PI or other labeling reagent for cell surfaces.
Analyze samples under a microscope or on a flow cytometer.
Annexin-V-Biotin Roche
xy1168 HCM 人心肌细胞 Human Cardiac Myocytes 5 x 10^5 cells/vial ATCC
xy1169 HCM-a 人心肌细胞--成人 Human Cardiac Myocytes-adult 5 x 10^5 cells/vial ATCC
xy1170 HCF 人心脏成纤维细胞 Human Cardiac Fibroblasts 5 x 10^5 cells/vial ATCC
xy1171 HCF-av 人心脏成纤维细胞--成人心室 Human Cardiac Fibroblasts-adult ventrical 5 x 10^5 cells/vial ATCC
xy1172 HCF-aa 人心脏成纤维细胞--成人心房 Human Cardiac Fibroblasts-adult atrial 5 x 10^5 cells/vial ATCC
xy1173 HCF-a 人心脏成纤维细胞--成人 Human Cardiac Fibroblasts-adult 5 x 10^5 cells/vial ATCC
xy1174 HCF-fa 人心脏成纤维细胞--胎儿心房 Human Cardiac Fibroblasts-fetal atrial 6 x 10^5 cells/vial ATCC
xy1175 HPcF 人心包成纤维细胞 Human Pericardial Fibroblasts 5 x 10^5 cells/vial ATCC
xy1176 ATCC
xy1177 视觉细胞系统 ATCC
xy1178 HCEpiC 人角膜上皮细胞 Human Corneal Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1179 HK 人角膜细胞 Human Keratocytes 5 x 10^5 cells/vial ATCC
xy1180 HREC 人视网膜内皮细胞 Human Retinal Endothelial Cells 5 x 10^5 cells/vial ATCC
xy1181 HRPEpiC 人视网膜色素上皮细胞 Human Retinal Pigment Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1182 HLEpiC 人晶状体上皮细胞 Human Lens Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1183 HIPEpiC 人虹膜色素上皮细胞 Human Iris Pigment Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1184 HConF 人结膜成纤维细胞 Human Conjunctival Fibroblasts 5 x 10^5 cells/vial ATCC
xy1185 HNPCEpiC 人无色素睫装上皮细胞 Human Non-Pigment Ciliary Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1186 HTMC 人小梁网细胞 Human Trabecular Meshwork Cells 5 x 10^5 cells/vial ATCC
xy1187 HOCF 人眼脉络膜成纤维细胞 Human Ocular Choroid Fibroblasts 5 x 10^5 cells/vial ATCC
xy1188 HConEpiC 人结膜上皮细胞 Human Conjunctival Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1189 ATCC
xy1190 胎盘细胞系统 ATCC
xy1191 HVCEC 人绒毛膜毛细血管内皮细胞 Human Villus Capillary Endothelial Cells 5 x 10^5 cells/vial ATCC
xy1192 HAmEpiC 人羊膜上皮细胞 Human Amniotic Epithelial Cells 5 x 10^5 cells/vial ATCC
xy1193 HVT 人绒毛膜滋养层细胞 Human Villous Trophoblasts 1 x 10^6 cells/vial ATCC
xy1194 HVMF 人绒毛间充质成纤维细胞 Human Villous Mesenchymal Fibroblasts 5 x 10^5 cells/vial ATCC
xy1195 脂肪细胞系统 ATCC
xy1196 HAMEC 人脂肪微血管内皮细胞 Human Adipose Microvascular Endothelial Cells 1 x 10^6 cells/vial ATCC
xy1197 HPA-v 人前脂肪细胞--内脏 Human Preadipocytes-visceral 1 x 10^6 cells/vial ATCC
xy1198 HPA-s 人前脂肪细胞--皮下 Human Preadipocytes-subcutaneous 1 x 10^6 cells/vial ATCC
xy1199 ATCC
xy1200 女性生殖细胞系统 ATCC
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文献和实验相关实验
Q：Annexin V 凋亡检测试剂盒的原理是什么？ A：Annexin V 是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸 PS 有高度亲和力，可通过细胞外侧暴露的 PS 与凋亡早期细胞胞膜结合，将 Annexin V 标记荧光染料如 FITC 利用流式细胞仪或荧光显微镜可检测细胞凋亡。 碘化丙啶(Propidium Iodide, PI)或 7-AAD 可与双链 DNA 特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，核染料 PI 或 7-AAD
示例： Jurkat细胞用1μM喜树碱(Camptothecin) (左)或未加药 (右)处理4h，FITC-Annexin V/PI荧光双染细胞凋亡检测试剂盒染色后，流式细胞仪荧光检测。 Annexin V-FITC单阳性细胞为早期凋亡细胞，Annexin V-FITC和PI染色双阳性的细胞为坏死细胞或者晚期凋亡。PI单染色阳性位裸核细胞。 实测数据可能会因细胞类型、细胞凋亡情况，检测仪器等的不同而存在差异，图中数据仅供参考。 流式检测细胞凋亡的数据分析方法： 1） 选中所有颗粒，将FSC
磷脂酰丝氨酸（Phosphatidylserine, PS）正常位于细胞膜的内侧，但在细胞凋亡的早期，PS可从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中（图3）。Annexin-V是一种分子量为35~36KD的Ca2+依赖性磷脂结合蛋白，能与PS高亲和力特异性结合。将Annexin-V进行荧光素（FITC、PE）或biotin标记，以标记了的Annexin-V作为荧光探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生。碘化丙啶（propidine iodide, PI）是一种核酸
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Annexin-V-Biotin Roche
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