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- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Methylamp Methylated DNA Capture Kit
- 库存：
1
- 供应商：
上海信裕
- 甲基化DNA获取试剂盒详细信息：
-
Product Overview
The Methylamp™ Methylated DNA Capture Kit is a complete set of essential components which enables the experimenter to use proprietary and unique procedure/compositions to enrich methylated DNA. In the assay, an antibody specific to methyl cytosine is used to capture methylated genomic DNA. The enriched methylated fractions can be then used for a standard DNA detection. The entire procedure can be completed within 3 hours and produces far superior results than any competitor kits. The Methylamp™ Methylated DNA Capture Kit is suitable for combining the specificity of enriched methylated DNA with qualitative and quantitative PCR, and southern blot as well as DNA microarray.
WHY CHOOSE THE METHYLAMP™ METHYLATED DNA CAPTURE KIT?- Highly efficient enrichment of methylated DNA: > 98%.
- The fastest procedure available, which can be finished within 3 hours.
- Strip microplate format makes the assay flexible: manual or high throughput.
- Columns for DNA purification are included: save time and reduce labor.
- Compatible with all DNA amplification-based approaches.
- Simple, reliable, and consistent assay conditions.
甲基化DNA获取试剂盒Principle & Procedure
The Methylamp™ Methylated DNA Capture Kit contains all reagents required for carrying out a successful capture of methylated DNA from a DNA sample. Particularly, this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA is sheared, added into the microwell and captured by the antibody. DNA is released from the antibody-captured methylated DNA complex, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
SCHEMATIC PROCEDURE: 
甲基化DNA获取试剂盒Product Components
MC1 (antibody buffer)
MC2 (Reaction buffer)
MC3 (wash buffer)
MC4 (DNA release buffer)
MC5 (binding buffer)
MC6 (elution buffer)
Normal mouse IgG (1 mg/ml)*
Anti-5-methylcytosine (1mg/ml)*
Proteinase K (10 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide
80924-4 大提柱式血液DNAout
80920-1 96孔板血液DNAout
80920-5 96孔板血液DNAout
71002-30 凝固血液DNAout
71002-100 凝固血液DNAout
71204-50 柱式凝固血液DNAout
80805-50 柱式毛发DNAout
91102-50 柱式骨骼DNAout
80801-50 柱式拭子DNAout
60501-20 一管式口腔拭子DNAout
60501-100 一管式口腔拭子DNAout
70502-30 石蜡包埋组织DNAout
70601-30 粪便DNAout
70601-100 粪便DNAout
80102-50 柱式粪便DNAout
80602-50 柱式凋亡DNAout
90314-50 柱式尿液DNAout
80803-50 柱式线粒体DNAout
81101-50 柱式昆虫DNAout
3672-100 植物DNAout
3672-250 植物DNAout
60602-50 柱式植物DNAout
80925-4 大提柱式植物DNAout
60705-50 一管式植物DNAout
60705-500 一管式植物DNAout
60805-20 中草药DNAout
60805-100 中草药DNAout
60304-50 真菌DNAout
60304-250 真菌DNAout
70901-50 柱式真菌DNAout
100303-50 玻璃珠法柱式真菌DNAout
80926-4 大提柱式真菌DNAout
* Spin the solution down to the bottom before use.甲基化DNA获取试剂盒Frequently Asked Qs
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文献和实验6.甲基化 DNA 免疫沉淀技术（MeDIP） 4.我国 DNA 甲基化检测产品获证情况 目前，基于 DNA 甲基化位点的检测产品陆续面世，给疾病诊断筛查带来福音。国内甲基化试剂盒转化商业产品集中在近几年，且获批的都为甲基化特异性 PCR 技术，主要原因还是该技术操作简便、特异性强、灵敏度高等特点。对于检测样本来说，目前相关的试剂盒检测样本类型多为 cfDNA ，特别是 ctDNA ，其检测方法具有早期、无创、快捷、灵敏度高等特点。 表 2. 国内NMPA获证情况
一、 什么是DNA甲基化在甲基转移酶的催化下，DNA的CG两个核苷酸的胞嘧啶被选择性地添加甲基，形成5－甲基胞嘧啶，这常见于基因的5'-CG-3'序列。大多数脊椎动物基因组DNA都有少量的甲基化胞嘧啶，主要集中在基因5'端的非编码区，并成簇存在。甲基化位点可随DNA的复制而遗传，?因为DNA复制后，甲基化酶可将新合成的未甲基化的位点进行甲基化。DNA 甲基化是表观遗传学的重要部分，同组蛋白修饰相互作用，通过改变染色质结构，调控基因表达。在哺乳类细胞或人体细胞中，DNA 甲基化与细胞的增殖、衰老
2.2.10 DNA微阵列法 Yan等2001年[40]将以分子杂交为基础的微阵列技术应用于DNA甲基化检测中，这种方法是基于杂交的寡核苷酸微阵列，是一种在基因组中寻找新位点的方法。包括用于整个基因组范围内扫描的差异甲基化（DMH）杂交（Huang等1999年[41]）和用于检测某个位点的甲基化特异性微阵列MSO（Gitan等2002年[42]）。前者类似于mRNA表达谱或cDNA微阵列，是CpG岛微阵列，后者类似于寡核苷酸微阵列，是针对CpG二核苷酸位点的甲基化特异性寡核苷酸微阵列[26
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