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- 2025年11月20日
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- 详细信息
- 技术资料
- 库存：
1
- 英文名：
Methylamp Methylated DNA Capture Kit
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 甲基化DNA获取试剂盒详细信息：
-
Product Overview
The Methylamp™ Methylated DNA Capture Kit is a complete set of essential components which enables the experimenter to use proprietary and unique procedure/compositions to enrich methylated DNA. In the assay, an antibody specific to methyl cytosine is used to capture methylated genomic DNA. The enriched methylated fractions can be then used for a standard DNA detection. The entire procedure can be completed within 3 hours and produces far superior results than any competitor kits. The Methylamp™ Methylated DNA Capture Kit is suitable for combining the specificity of enriched methylated DNA with qualitative and quantitative PCR, and southern blot as well as DNA microarray.
WHY CHOOSE THE METHYLAMP™ METHYLATED DNA CAPTURE KIT?- Highly efficient enrichment of methylated DNA: > 98%.
- The fastest procedure available, which can be finished within 3 hours.
- Strip microplate format makes the assay flexible: manual or high throughput.
- Columns for DNA purification are included: save time and reduce labor.
- Compatible with all DNA amplification-based approaches.
- Simple, reliable, and consistent assay conditions.
甲基化DNA获取试剂盒Principle & Procedure
The Methylamp™ Methylated DNA Capture Kit contains all reagents required for carrying out a successful capture of methylated DNA from a DNA sample. Particularly, this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA is sheared, added into the microwell and captured by the antibody. DNA is released from the antibody-captured methylated DNA complex, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
SCHEMATIC PROCEDURE: 甲基化DNA获取试剂盒Product Components
MC1 (antibody buffer)
MC2 (Reaction buffer)
MC3 (wash buffer)
MC4 (DNA release buffer)
MC5 (binding buffer)
MC6 (elution buffer)
Normal mouse IgG (1 mg/ml)*
Anti-5-methylcytosine (1mg/ml)*
Proteinase K (10 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide
80924-4 大提柱式血液DNAout
80920-1 96孔板血液DNAout
80920-5 96孔板血液DNAout
71002-30 凝固血液DNAout
71002-100 凝固血液DNAout
71204-50 柱式凝固血液DNAout
80805-50 柱式毛发DNAout
91102-50 柱式骨骼DNAout
80801-50 柱式拭子DNAout
60501-20 一管式口腔拭子DNAout
60501-100 一管式口腔拭子DNAout
70502-30 石蜡包埋组织DNAout
70601-30 粪便DNAout
70601-100 粪便DNAout
80102-50 柱式粪便DNAout
80602-50 柱式凋亡DNAout
90314-50 柱式尿液DNAout
80803-50 柱式线粒体DNAout
81101-50 柱式昆虫DNAout
3672-100 植物DNAout
3672-250 植物DNAout
60602-50 柱式植物DNAout
80925-4 大提柱式植物DNAout
60705-50 一管式植物DNAout
60705-500 一管式植物DNAout
60805-20 中草药DNAout
60805-100 中草药DNAout
60304-50 真菌DNAout
60304-250 真菌DNAout
70901-50 柱式真菌DNAout
100303-50 玻璃珠法柱式真菌DNAout
80926-4 大提柱式真菌DNAout
* Spin the solution down to the bottom before use.甲基化DNA获取试剂盒Frequently Asked Qs
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甲基化DNA获取试剂盒
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