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- 文献和实验
- 技术资料
- 保存条件:
4℃保存
- 保质期:
6个月
- 英文名:
EpiQuik Tissue Methyl-Histone H3-K9 ChIP Kit
- 库存:
1
- 供应商:
上海信裕
- 组织甲基组蛋白H3-K9免疫沉淀试剂盒详细信息:
-
Product Overview
The EpiQuik™ Tissue Methyl-Histone H3-K9 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3-K9 methylation and DNA in a variety of mammalian tissues. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tissue Methyl-Histone H3-K9 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.
WHY CHOOSE THE EPIQUIK™ TISSUE METHYL-HISTONE H3-K9 ChIP KIT?- The fastest procedure available, which can be finished within 5 hours.
- Strip microwell format makes the assay flexible: manual or high throughput.
- Columns for DNA purification are included: save time and reduce labor.
- Compatible with all DNA amplification-based approaches.
- Simple, reliable, and consistent assay conditions.
组织甲基组蛋白H3-K9免疫沉淀试剂盒Principle & Procedure
The EpiQuik™ Tissue Methyl-Histone H3-K9 ChIP Kit contains all reagents necessary for carrying out a successful chromatin immunoprecipitation for methyl-histone H3-K9 from mammalian tissues. Particularly, this kit includes a ChIP-grade methyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured methyl-histone H3-K9 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
SCHEMATIC PROCEDURE:
组织甲基组蛋白H3-K9免疫沉淀试剂盒Product Components
CP1 (wash buffer)
CP2 (antibody buffer)
CP3 (lysis buffer)
CP4 (CHIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Homogenizing buffer
Protease inhibitor cocktail (100x)*
Proteinase K (10 mg/ml)*
Normal mouse IgG (0.5 mg/ml)*
Anti-dimethyl-H3-K9 (1 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide
xy103-3 兔 IgG
xy103-4 兔 IgG
xy104-1 兔 IgG ( 液体-Ph 7.2 PBS )
xy104-2 兔 IgG ( 液体-Ph 7.2 PBS )
xy104-3 兔 IgG ( 液体-Ph 7.2 PBS )
xy105-1 羊 IgG
xy105-2 羊 IgG
xy105-3 羊 IgG
xy105-4 羊 IgG
xy106-1 羊 IgG( 液体 )
xy106-2 羊 IgG( 液体 )
xy106-3 羊 IgG( 液体 )
xy107-1 小鼠 IgG
xy107-2 小鼠 IgG
xy107-3 小鼠 IgG
xy108-1 小鼠 IgG (液体-Ph 7.2 PBS)
xy108-2 小鼠 IgG (液体-Ph 7.2 PBS)
xy109-1 大鼠 IgG
xy109-2 大鼠 IgG
xy109-3 大鼠 IgG
xy110-1 大鼠 IgG (液体-Ph 7.2 PBS)
xy110-2 大鼠 IgG (液体-Ph 7.3 PBS)
xy111-1 豚鼠 IgG
xy111-2 豚鼠 IgG
xy112-1 豚鼠 IgG (液体-Ph 7.2 PBS)
xy112-2 豚鼠 IgG (液体-Ph 7.2 PBS)
xy113-1 猪 IgG
xy113-2 猪 IgG
xy113-3 猪 IgG
xy113-4 猪 IgG
xy114-1 猪 IgG( 液体-Ph 7.2 PBS )
xy114-2 猪 IgG( 液体-Ph 7.3 PBS )
xy114-3 猪 IgG( 液体-Ph 7.4 PBS )
* Spin the solution down to the bottom before use.组织甲基组蛋白H3-K9免疫沉淀试剂盒User Guide & MSDS
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文献和实验9.19 Nature:揭示组蛋白标记 H3K36me2 招募 DNMT3A 并影响基因间 DNA 甲基化
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NCB:张勇 / 高绍荣 / 刘文强合作揭示小鼠早期胚胎发育中潜在的印记控制区域
在哺乳动物发育过程中,DNA 甲基化、组蛋白修饰等表观遗传修饰,在父母源基因组经历了不同模式的重编程。一些亲本间差异的表观遗传修饰能够逃脱早期胚胎发育过程的重编程,其中最典型的是印记控制区域(imprinting control regions, ICRs)。ICR 在胚胎发育过程中发挥重要作用,其表观遗传修饰的异常会导致胚胎发育的异常。目前在小鼠中仅发现 21 个 ICR,识别新的亲本表观遗传特异的功能性区域面临如下挑战:依赖于品系间单核苷酸多态性(SNP)识别亲本间表观遗传异质性的识别
家族 (KRAB-ZFPs)。这两个 标记均在失活的 X 染色体上发现,其中,H3K27me3 位于基因间和沉默的编码区,H3K9me3 主要位 于活性基因的编码区。 酶调节 组蛋白甲基化是一种可以通过多次细胞分裂而仍然保持的稳定标记,多年来一直被认为不可逆。但是, 最新研究发现,它是一个主动调控和可逆的过程。 甲基化:组蛋白甲基转移酶 (HMTs) 赖氨酸 包含 SET 结构域(组蛋白尾部) 包含非 SET 结构域(组蛋白核心) 精氨酸 PRMT(蛋白
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