组织甲基组蛋白H3-K9免疫沉淀试剂盒

组织甲基组蛋白H3-K9免疫沉淀试剂盒

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  • 美国eBioscince
  • 美国/中国
  • xy-2008
  • 2025年07月14日
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    • 详细信息
    • 技术资料
    • 保存条件

      4℃保存

    • 保质期

      6个月

    • 英文名

      EpiQuik Tissue Methyl-Histone H3-K9 ChIP Kit

    • 库存

      1

    • 供应商

      上海信裕

    • 组织甲基组蛋白H3-K9免疫沉淀试剂盒详细信息:
    • Product Overview

      The EpiQuik™ Tissue Methyl-Histone H3-K9 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3-K9 methylation and DNA in a variety of mammalian tissues. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tissue Methyl-Histone H3-K9 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.

      WHY CHOOSE THE EPIQUIK™ TISSUE METHYL-HISTONE H3-K9 ChIP KIT?
      • The fastest procedure available, which can be finished within 5 hours.
      • Strip microwell format makes the assay flexible: manual or high throughput.
      • Columns for DNA purification are included: save time and reduce labor.
      • Compatible with all DNA amplification-based approaches.
      • Simple, reliable, and consistent assay conditions.
      组织甲基组蛋白H3-K9免疫沉淀试剂盒Principle & Procedure


      The EpiQuik™ Tissue Methyl-Histone H3-K9 ChIP Kit contains all reagents necessary for carrying out a successful chromatin immunoprecipitation for methyl-histone H3-K9 from mammalian tissues. Particularly, this kit includes a ChIP-grade methyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured methyl-histone H3-K9 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

      SCHEMATIC PROCEDURE:
      组织甲基组蛋白H3-K9免疫沉淀试剂盒

      组织甲基组蛋白H3-K9免疫沉淀试剂盒Product Components


      CP1 (wash buffer)
      CP2 (antibody buffer)
      CP3 (lysis buffer)
      CP4 (CHIP dilution buffer)
      CP5 (DNA release buffer)
      CP6 (reverse buffer)
      CP7 (binding buffer)
      CP8 (elution buffer)
      Homogenizing buffer
      Protease inhibitor cocktail (100x)*
      Proteinase K (10 mg/ml)*
      Normal mouse IgG (0.5 mg/ml)*
      Anti-dimethyl-H3-K9 (1 mg/ml)*
      8-well assay strips (with frame)
      8-well strip caps
      F-spin column
      F-collection tube
      User guide
      xy103-3 兔 IgG
      xy103-4 兔 IgG
      xy104-1 兔 IgG ( 液体-Ph 7.2 PBS )
      xy104-2 兔 IgG ( 液体-Ph 7.2 PBS )
      xy104-3 兔 IgG ( 液体-Ph 7.2 PBS )
      xy105-1 羊 IgG
      xy105-2 羊 IgG
      xy105-3 羊 IgG
      xy105-4 羊 IgG
      xy106-1 羊 IgG( 液体 )
      xy106-2 羊 IgG( 液体 )
      xy106-3 羊 IgG( 液体 )
      xy107-1 小鼠 IgG
      xy107-2 小鼠 IgG
      xy107-3 小鼠 IgG
      xy108-1 小鼠 IgG (液体-Ph 7.2 PBS)
      xy108-2 小鼠 IgG (液体-Ph 7.2 PBS)
      xy109-1 大鼠 IgG
      xy109-2 大鼠 IgG
      xy109-3 大鼠 IgG
      xy110-1 大鼠 IgG (液体-Ph 7.2 PBS)
      xy110-2 大鼠 IgG (液体-Ph 7.3 PBS)
      xy111-1 豚鼠 IgG
      xy111-2 豚鼠 IgG
      xy112-1 豚鼠 IgG (液体-Ph 7.2 PBS)
      xy112-2 豚鼠 IgG (液体-Ph 7.2 PBS)
      xy113-1 猪 IgG
      xy113-2 猪 IgG
      xy113-3 猪 IgG
      xy113-4 猪 IgG
      xy114-1 猪 IgG( 液体-Ph 7.2 PBS )
      xy114-2 猪 IgG( 液体-Ph 7.3 PBS )
      xy114-3 猪 IgG( 液体-Ph 7.4 PBS )
      * Spin the solution down to the bottom before use.

      组织甲基组蛋白H3-K9免疫沉淀试剂盒User Guide & MSDS

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