The EpiQuik™ Tissue Methyl-Histone H3-K9 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3-K9 methylation and DNA in a variety of mammalian tissues. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tissue Methyl-Histone H3-K9 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.
WHY CHOOSE THE EPIQUIK™ TISSUE METHYL-HISTONE H3-K9 ChIP KIT?
The fastest procedure available, which can be finished within 5 hours.
Strip microwell format makes the assay flexible: manual or high throughput.
Columns for DNA purification are included: save time and reduce labor.
Compatible with all DNA amplification-based approaches.
Simple, reliable, and consistent assay conditions.
组织甲基组蛋白H3-K9免疫沉淀试剂盒Principle & Procedure
The EpiQuik™ Tissue Methyl-Histone H3-K9 ChIP Kit contains all reagents necessary for carrying out a successful chromatin immunoprecipitation for methyl-histone H3-K9 from mammalian tissues. Particularly, this kit includes a ChIP-grade methyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured methyl-histone H3-K9 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.