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Methylamp DNA Modification Kit
1
上海信裕
The Methylamp™ DNA Modification Kit is a complete set of essential components which enables the experimenter to perform DNA methylation analysis using Epigenteks uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within a mere 1 hour and 55 minutes and produces far superior results than any competitor kits. The Methylamp™ DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
WHY CHOOSE THE METHYLAMP™ DNA MODIFICATION KIT?
The Methylamp™ DNA Modification Kit contains all reagents required for bisulfite conversion on a DNA sample. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to bind tightly to the column filter, thus clean DNA can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.
High throughput version also available. [Cat. # P-1008]
Fast DNA Modification version using heating process also available. [Cat. # P-1010]
SCHEMATIC PROCEDURE: | COMPARATIVE OVERVIEW: |
The different amounts of DNA isolated from a serum sample were chemically modified using the Methylamp™ DNA Modification Kit. Real time PCR was performed by using a pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin. |
KIT CONTENTS | 40 samples P-1001-1 |
80 samples P-1001-2 |
R1 (DNA denature) | 0.25 ml | 0.5 ml |
R2 (DNA modification) | 4 vials | 8 vials |
R3 (DNA modification) | 5 ml | 10 ml |
R4 (modified DNA capture) | 14 ml | 28 ml |
R5 (modified DNA cleaning) | 3 ml | 6 ml |
R6 (modified DNA elution) | 1 ml | 2 ml |
F-spin column | 40 | 80 |
F-collection tube | 40 | 80 |
User guide | 1 | 1 |
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