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- 2025年07月10日
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- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
Methylamp DNA Modification Kit
- 库存：
1
- 供应商：
上海信裕
- DNA甲基化修饰试剂盒详细信息：
-
Product Overview
The Methylamp™ DNA Modification Kit is a complete set of essential components which enables the experimenter to perform DNA methylation analysis using Epigenteks uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within a mere 1 hour and 55 minutes and produces far superior results than any competitor kits. The Methylamp™ DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
WHY CHOOSE THE METHYLAMP™ DNA MODIFICATION KIT?
- The fastest procedure available on the market, which can be completed within 1 hour and 55 minutes with consistent reaction conditions.
- Completely converts unmethylated cytosine into uracil: modified DNA > 99.98%.
- The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented.
- The lowest requirement of starting DNA for modification: only 50 pg or 20 cells.
- Extremely simple, reliable, and consistent modification conditions.
DNA甲基化修饰试剂盒Principle & Procedure
The Methylamp™ DNA Modification Kit contains all reagents required for bisulfite conversion on a DNA sample. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to bind tightly to the column filter, thus clean DNA can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.
High throughput version also available. [Cat. # P-1008]
Fast DNA Modification version using heating process also available. [Cat. # P-1010]
SCHEMATIC PROCEDURE: COMPARATIVE OVERVIEW: 
The different amounts of DNA isolated from a serum sample were chemically modified using the Methylamp™ DNA Modification Kit. Real time PCR was performed by using a pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin.DNA甲基化修饰试剂盒Product ComponentsKIT CONTENTS 40 samples
P-1001-180 samples
P-1001-2R1 (DNA denature) 0.25 ml 0.5 ml R2 (DNA modification) 4 vials 8 vials R3 (DNA modification) 5 ml 10 ml R4 (modified DNA capture) 14 ml 28 ml R5 (modified DNA cleaning) 3 ml 6 ml R6 (modified DNA elution) 1 ml 2 ml F-spin column 40 80 F-collection tube 40 80 User guide 1 1 DNA甲基化修饰试剂盒Frequently Asked Qs
1. What is difference between one-step DNA modification and two-step DNA modification?
In the one-step DNA modification, DNA is denatured by heating, which allows DNA denaturation and bisulfite modification to be carried out simultaneously. One-step DNA modification is suitable for the labs which are equipped with thermal cycler and requires simple and fast procedures for DNA modification. In the two-step DNA modification, DNA is denatured chemically followed by bisulfite treatment. It is suitable for general DNA modification using DNA isloated from various sources. Because one-step DNA modification may increase DNA degradation, a higher starting DNA amount may be required for one-step DNA modification than for two-step DNA modifcation.
2. How much starting DNA is required for DNA modification with this kit?
The starting DNA required for DNA modification can be as low as 50 pg. The signal of the modified DNA can be detected with real-time PCR.
3. Why are only 90 minutes required for the actual DNA modification?
With our unique modification composition, 90 minutes is sufficient for more than 99% C-T conversion while DNA degradation is greatly prevented. We have observed that increase in modification time did not significantly increase C-T conversion, while yield of modified DNA was significantly reduced most likely due to increased DNA degradation.
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xy-E10069 L-Carnitine ELISA试剂盒 检测范围
xy-E10070 小反刍兽疫抗体(PPR Ab)ELISA试剂盒 检测范围
xy-E10071 副结核分枝杆菌抗体(M.pt Ab)ELISA试剂盒 检测范围
xy-E10072 结核分枝杆菌抗体（M.tuberculosis Ab）ELISA试剂盒 检测范围
xy-E10073 可溶性血管内皮生长因子受体2(VEGFR-2/sFLK-1)ELISA试剂盒 检测范围
xy-E10074 胸腺基质淋巴细胞生成素(TSLP)ELISA试剂盒 检测范围
xy-E10075 穿孔素/成孔蛋白(PF/PFP)ELISA试剂盒 检测范围
xy-E10076 多效生长因子(PTN)ELISA试剂盒 检测范围
xy-E10077 可溶性CD28(sCD28)ELISA试剂盒 检测范围
xy-E10078 淋巴细胞因子ELISA试剂盒 检测范围
xy-E10079 胸腺活化调节趋化因子(TARC/CCL17)ELISA试剂盒 检测范围
xy-E10080 神经细胞粘附分子配体1(NCAM-L1/CD171)ELISA试剂盒 检测范围
xy-E10081 神经保护因子(CVNPF)ELISA试剂盒 检测范围
xy-E10082 可溶性肿瘤坏死因子α受体(sTNFαR)ELISA试剂盒 检测范围
xy-E10083 可溶性细胞因子受体(sCKR)ELISA试剂盒 检测范围
xy-E10084 可溶性凋亡相关因子配体(sFASL)ELISA试剂盒 检测范围
xy-E10085 细胞凋亡抑制因子(IAP)ELISA试剂盒 检测范围
xy-E10086 集落刺激因子(CSF)ELISA试剂盒 检测范围
xy-E10087 γ干扰素诱导单核细胞因子(MIGF/CXCL9)ELISA试剂盒 检测范围
xy-E10088 干扰素诱导T细胞趋化因子(ITAC/CXCL11)ELISA试剂盒 检测范围
xy-E10089 CD14分子(CDl4)ELISA试剂盒 检测范围
xy-E10090 凋亡诱导因子(AIF)ELISA试剂盒 检测范围
xy-E10091 白细胞共同抗原(LCA/CD45)ELISA试剂盒 检测范围
xy-E10092 CD4分子(CD4)ELISA试剂盒 检测范围
xy-E10093 P钙黏蛋白/胎盘钙黏蛋白(P-cad)ELISA试剂盒 检测范围
xy-E10094 角化细胞生长因子(KGF)ELISA试剂盒 检测范围
xy-E10095 血小板衍生生长因子BB(PDGF-BB)ELISA试剂盒 检测范围
xy-E10096 CXC趋化因子配体16(CXCL16)ELISA试剂盒 检测范围
xy-E10097 CXC趋化因子受体3(CXCR3)ELISA试剂盒 检测范围
xy-E10098 γ干扰素诱导蛋白16/p16(IFI16/p16)ELISA试剂盒 检测范围
xy-E10099 基质细胞衍生因子1a(SDF-1a/CXCL12)ELISA试剂盒 检测范围
xy-E10100 淋巴细胞趋化因子(Lptn/LTN/XCL1)ELISA试剂盒 检测范围
4. Can the modified DNA with this kit be stored for a long time?
The modified DNA generated with this kit can be stored for 2 months at -20°C. The modified DNA could be stored for as long as 6 months at -80°C.
5. Can the kit be used for modifying DNA from formalin-fixed and paraffin-embedded (FFPE) samples?
DNA extracted from FFPE samples is often fragmented. The kit can be used for FFPE samples as the modification solution included in the kit contains DNA stabilizing reagents that avoid a further fragmentation of DNA caused in modification process.User Guide & MSDS
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组蛋白修饰和 DNA 甲基化之间的关系 在引起基因 沉默的过程中，沉默信号(DNA甲基化、组蛋白修饰、染色质重新装配)是如何进行的?谁先谁后?这是一个“鸡和蛋”的问题，目前仍处于研究阶段，还没有定论。研究发现DNA甲基化和组蛋白乙酰化是一个相互促进、加强的过程，如许多HDAC可以和DNMTl、3a、3b相互作用；而甲基化CpG结合蛋白―2(methylcytosinebindingprotein―2，MeCP―2)又可以和HDAC相互作用。这种作用方式提示
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