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上海玉博生物科技有限公司
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文献和实验Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
of H3K9me2, but not H3K9me3, in heterochromatin of Arabidopsis , while H3K9me3, but not H3K9me2, is observed in heterochromatin of mice [21,24,25]. Controls for ChIP To ensure the reliability of ChIP data, two control samples specific for the ChIP
that was an interspecific hybrid (H) between Mus musculus domesticus (D, paternal genome) and Mus spretus (S, maternal genome). The native chromatin was then immunoprecipitated with rabbit polyclonal antisera to acetylation at lysine 9 of H3 (H3K9Ac) and to dimethylation
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