- 询价
- ATCC
- 美国/中国
- xy-12186
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
上海信裕
- 细胞形态：
上皮样
- 运输方式：
干冰运输
- 生长状态：
贴壁生长
产品信息
别称：
CXCL3, GRO3, GROG, SCYB3
蛋白构建：
A DNA sequence encoding the human CXCL3 (NP_002081.2) (Ala35-Asn107) was expressed with a polyhistidine tag at the N-terminus.
种属：
Human
表达宿主：
E. coli
人CXCL3/GRO gamma蛋白(isoform a，His标签)产品状态&运输说明：
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
质控检测
纯度：
> 90 % as determined by SDS-PAGE.
内毒素：
Please contact us for more information.
稳定性：
Samples are stable for up to twelve months from date of receipt at -70℃
预测N端：
His
人CXCL3/GRO gamma蛋白(isoform a，His标签)分子量：
The recombinant human CXCL3 consists of 91 amino acids and predicts a molecular mass of 10.1 KDa. It migrates as an approximately 11-15 KDa band in SDS-PAGE under reducing conditions.
缓冲液：
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
使用指导
储存方法：
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件：
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.人CXCL3/GRO gamma蛋白(isoform a，His标签)
xy1747 XO "黄嘌呤氧化酶检测试剂盒
" Xanthine Oxidase Assay 100 Tests in 96-well plat
xy1748 TGA 总谷胱甘肽含量检测试剂盒 Total Glutathione Assay 100 tests
xy1749 干细胞检测试剂盒
xy1750 Ared 茜素红S染色试剂盒 Alizarin Red S Staining Kit 100 ml
xy1751 HGL PCR 人胚层检测试剂盒 Human Germ Layer Detection Kit 50 reactions
xy1752 Ored 油红O染色试剂盒 Oil Red O Staining Kit 100 ml
xy1753 HSC PCR 人干细胞多能性检测试剂盒 Human Stem Cell Pluripotency Detection Kit 50 reactions
xy1754 ALP 碱性磷酸酶活性测定试剂盒 Alkaline Phosphatase Activity Assay 500 tests
xy1755 ALPr 碱性磷酸酶染色检测（红色） Alkaline Phosphatase Staining Assay (Red) 500 tests
xy1756 PCR 人间质干细胞成脂肪细胞检测试剂盒 Human Mesenchymal Stem Cell Adipogenesis Detection Kit 50 reactions
xy1757 HMSC-C PCR 人间充质干细胞软骨形成检测试剂盒 Human Mesenchymal Stem Cell Chondrogenesis Detection Kit 50 reactions
xy1758 SafraninO 番红O染色试剂盒 Safranin O Staining Kit 100 reactions
xy1759 ABlue 阿利新蓝染色试剂盒 Alcian Blue Staining Kit 100 reactions
xy1760
xy1761
xy1762 hActA-ELISA 人活化素A酶联免疫试剂盒 Human Activin A ELISA Kit 96 tests
xy1763 hANG-ELISA 人双调蛋白酶联免疫试剂盒 Human ANG ELISA Kit 96 tests
xy1764 hBDNF-ELISA 人脑源性神经生长因子酶联免疫试剂盒 Human BDNF ELISA Kit 96 tests
xy1765 hBMP-5-ELISA 人BMP-5酶联免疫试剂盒 Human BMP-5 ELISA Kit 96 tests
xy1766 hBMP-2-ELISA 人BMP-2酶联免疫试剂盒 Human BMP-2 ELISA Kit 96 tests
xy1767 hBMP-4-ELISA 人BMP-4酶联免疫试剂盒 Human BMP-4 ELISA Kit 96 tests
xy1768 hEGF-ELISA 人表皮生长因子酶联免疫试剂盒 Human EGF ELISA Kit 96 tests
xy1769 hEGFR-ELISA 人表皮生长因子受体酶联免疫试剂盒 Human EGFR ELISA Kit 96 tests
xy1770 hFAS-ELISA 人凋亡相关因子酶联免疫试剂盒 Human FAS ELISA Kit 96 tests
xy1771 hFASL-ELISA 人凋亡相关因子配体酶联免疫试剂盒 Human FASL ELISA Kit 96 tests
xy1772 hFGF-9-ELISA 人FGF-9酶联免疫酶联免疫试剂盒 Human FGF-9 ELISA Kit 96 tests
xy1773 hFN-ELISA 人纤维连接蛋白酶联免疫试剂盒 Human Fibronectin ELISA Kit 96 tests
xy1774 hG-CSF-ELISA "人粒细胞集落刺激因子酶联免疫试剂盒
" Human G-CSF ELISA Kit 96 tests
xy1775 hGDNF-ELISA 人神经胶质细胞源性的神经营养因子酶联免疫试剂盒 Human GDNF ELISA Kit 96 tests
xy1776 hGM-CSF-ELISA "人粒细胞巨噬细胞刺激因子酶联免疫试剂盒
" Human GM-CSF ELISA Kit 96 tests
xy1777 hHGF-ELISA 人肝细胞生长因子酶联免疫试剂盒 Human HGF ELISA Kit 96 tests
xy1778 hICAM-1-ELISA 人细胞间粘附分子-1酶联免疫试剂盒 Human ICAM-1 ELISA Kit 96 tests
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文献和实验3.表达序列标签（EST）的研究现状 4.http://www.bbioo.com/bio101/2007/20990.htm otolaryngology To zhujoker：非常感谢，人类的基因序列和其中一种异构体蛋白序列已经完成，至于有没有isoform和几种isoform都不知道；小鼠的DNA是知道的，但是在ncbi中显示小鼠的mRNA序列尚未完全标注，其他的物种DNA都没有测完，从DNA及ensembl预测的mRNA序列来看是比较保守
His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
Low (“Low”)Imidazole Buffer 0.5L 100mM Imidizole 3.4g 5% glycerol 25ml 100% glycerol 50mM Tris-HCl (pH 7.9)50ml 0.5M Tris-HCl (pH 7.9) 0.1% Tween-20 0.5M 100% Tween-20 500mM NaCl50ml 5M NaCl dH2O Fill to 0.5L (start w/ 350ml) High (“High
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