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- 文献和实验
- 技术资料
- 库存:
1
- 供应商:
上海信裕
- 细胞形态:
上皮样
- 运输方式:
干冰运输
- 生长状态:
贴壁生长
产品信息
别称:
CCL3, Mip1a, Scya3
蛋白构建:
A DNA sequence encoding human CCL3 (NP_002974.1) (Ala23-Ala92) was expressed with a polyhistidine tag at the C-terminus.
种属:
Human
表达宿主:
Yeast
人CCL3蛋白 (His标签)产品状态&运输说明:
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
质控检测
纯度:
> 95 % as determined by SDS-PAGE.
内毒素:
Please contact us for more information.
人CCL3蛋白 (His标签)稳定性:
Samples are stable for up to twelve months from date of receipt at -70℃
预测N端:
Ala 23
分子量:
The recombinant human CCL3 consists of 80 amino acids and predicts a molecular mass of 9.2 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
缓冲液:
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
使用指导
储存方法:
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶条件:
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.人CCL3蛋白 (His标签)
xy1385 CoEpiCM 结肠上皮细胞培养基 Colonic Epithelial Cell Medium 500 ml ATCC
xy1386 BEpiCM 支气管上皮细胞培养基 Bronchial Epithelial Cell Medium 500 ml ATCC
xy1387 SAEpiCM 气管上皮细胞培养基 Small Airway Epithelial Cell Medium 500 ml ATCC
xy1388 EpiCM-2 上皮细胞培养基-2 Epithelial Cell Medium-2 500 ml ATCC
xy1389 UCM 尿道上皮细胞培养基 Urothelial Cell Medium 500 ml ATCC
xy1390 PEpiCM 前列腺上皮细胞培养基 Prostate Epithelial Cell Medium 500 ml ATCC
xy1391 STEMium 人胚胎干细胞无血清培养基 Human Pluripotent Stem Cell Growth Medium 500 ml ATCC
xy1392 STEMium-XF 无异种人胚胎干细胞生长培养基 Xeno-free Human Pluripotent Stem Cell Growth Medium 500 ml ATCC
xy1393 MEF-cm 小鼠胚胎成纤维细胞培养基 Mouse Embryonic Fibroblasts-Conditioned Medium 100 ml ATCC
xy1394 bFGF-std MEF-cm 碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基 bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium 100ml ATCC
xy1395 CEpiCM 角膜上皮细胞培养基 Corneal Epithelial Cell Medium 500 ml ATCC
xy1396 OEpiCM 卵巢上皮细胞培养基 Ovarian Epithelial Cell Medium 500 ml ATCC
xy1397 MSCM-sf 间充质干细胞培养基 Mesenchymal Stem Cell Medium-serum free 500 ml ATCC
xy1398 MEpiCM 乳腺上皮细胞培养基 Mammary Epithelial Cell Medium 500 ml ATCC
xy1399 ATCC
xy1400 ATCC
xy1401 KM-acf 角质细胞培养基 Keratinocyte Medium-animal component free 500 ml ATCC
xy1402 FM-acf 成纤维细胞培养基 Fibroblast Medium-animal component free 500 ml ATCC
xy1403 STEMium-ACF 人类多能干细胞培养基-无动物组分 STEMium. Human Pluripotent Stem Cell Growth Medium-animal component free 500ml ATCC
xy1404 MSCM-acf 间充质干细胞培养基 Mesenchymal Stem Cell Medium-animal component free 500 ml ATCC
xy1405 ATCC
xy1406 ATCC
xy1407 MEM MEM MEM with Earle's Salts and L-Glutamine 500 ml ATCC
xy1408 MEM MEM MEM with Hank's Salts and L-Glutamine 500 ml ATCC
xy1409 MEM MEM MEM with Eagles's Salts and Glutamine 500 ml ATCC
xy1410 M199E M199E Medium 199 with Earle's Salts and L-Glutamine 500 ml ATCC
xy1411 M199H M199H Medium 199 with Hanks Salts and L-Glutamine 500 ml ATCC
xy1412 DMEM DMEM DMEM with L-Glutamine and Sodium Pyruvate 500 ml ATCC
xy1413 DMEM DMEM DMEM with High-Glucose - L-Glutamine and Sodium Pyruvate 500 ml ATCC
xy1414 DMEM DMEM DMEM with High-Glucose - L-Glutamine and Sodium Pyruvate 1L ATCC
xy1415 DMEM DMEM DMEM with L-Glutamine - Sodium Pyruvate and 25 mM HEPES 500 ml ATCC
xy1416 DMEM DMEM DMEM with High-Glucose - L-Glutamine - Sodium Pyruvate and 25 mM HEPES 500 ml ATCC
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文献和实验His 标签蛋白纯化效果不理想,宝宝心里苦呀。今天,小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面: 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高,容易导致蛋白炭化;功率过低,蛋白释放不出来。 建议 改变超声功率,同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素,如:金属离子螯合剂、强还原剂、过高的咪唑浓度。同时,优化缓冲液的pH、盐
Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
Low (“Low”)Imidazole Buffer 0.5L 100mM Imidizole 3.4g 5% glycerol 25ml 100% glycerol 50mM Tris-HCl (pH 7.9)50ml 0.5M Tris-HCl (pH 7.9) 0.1% Tween-20 0.5M 100% Tween-20 500mM NaCl50ml 5M NaCl dH2O Fill to 0.5L (start w/ 350ml) High (“High
行 HIS 标签的捕获。 2、HIS标签蛋白结合了,但是洗脱不充分 原因一 洗脱条件太温和。 建议 增加咪唑的浓度或选择咪唑线性梯度洗脱,或者通过降低 pH 寻找最佳洗脱条件。需要注意的是,pH 低于 3.5 容易导致镍离子脱落。 原因二 蛋白柱上沉淀。 建议 1 降低蛋白浓度。可以减少上样量,或者采用咪唑线性而非步级梯度洗脱,增加蛋白稀释度。 建议 2 试用去污剂或者改变 NaCl 的浓度,或者使用变性剂 (4-6M 盐酸胍或 4-8M 尿素) 在变性条件下洗脱。 原因三 非特异性的疏水或者其他相互
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