- ¥1980
- SignaGen
- 美国
- SL100568-U2OS
- 2025年12月08日
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- 详细信息
- 技术资料
- 保存条件：
4°C
- 保质期：
1年
- 英文名：
GenMute™ siRNA Transfection Reagent for U2OS Cell
- 库存：
现货 大量
- 供应商：
美中清水湾生物科技有限公司
- 规格：
毫升
Description:
GenMute™ siRNA Transfection Reagent for U2OS Cell is pre-optimized for transfecting siRNA to U2OS cells. The U2OS cell line, originally known as the 2T line, was cultivated from the bone tissue of a fifteen-year-old human female suffering from osteosarcoma. Established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2OS cells exhibit epithelial adherent morphology and viruses were not detected in the line during co-cultivation with WI-38 cells or in CF tests against SV40, RSV, or adenoviruses. Cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).
Size & content:
- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 15 pmoles siRNA or miRNA mimics in 24-well plate
- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on U2OS cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: |
|
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol .pdf
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