- 询价
- SignaGen
- 美国
- SignaGen
- 2026年01月03日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
1年
- 库存：
现货 大量
- 供应商：
美中清水湾生物科技有限公司
SignaGen launches Adeno-Ready™ open reading frame (ORF) repository. The Adeno-Ready™ ORF repository contains more than 37,430 sequence-verified human ORFs, which were pre-cloned into our Ad·MAX™ adenovirus production system and ready to package into adenovirus. Most of Adeno-Ready™ ORFs have three different ends-------closed (no fusion tag) or tagged with V5 epitope or 6xHN tag (Figure 1). Based on these Adeno-Ready™ ORFs, we are offering a faster yet economical way to produce adenoviruses.
Figure 1. A cartoon showing that ORFs were pre-cloned into Ad·MAX adenovirus expression & production system with three different ends.
To make custom adenovirus, you only need to search the Adeno-Ready™ ORFs by NCBI accession #, gene symbol or GI number, identify the adenoviral clones and add the ready to package adenovirus into shopping card followed by online checkout. You will be receiving the purified adenovirus (1E+10~1E+11 PFU/ml via genuine PFU titration) with standard 4~5 weeks turnaround time. With adenoviral vectors, you can eventually efficiently transduce any mammalian cells dividing or non-dividing.
Features of Ready-to-package Adenoviruses:
- Adeno-Ready™ ORFs allows to make custom adenovirus with just a NCBI accession #
- Vast selection – over 37,430 Adeno-Ready™ human ORF clones
- Ready to Package Format – All ORFs were pre-cloned into our proprietary Ad-MAX™ adenovirus production system and ready to package
- 100% amino acid match guarantee – sequence verification against GenBank and Swissprot databases
- Unbeatable affordability with less than $800 per adenovirus
- Quick adenoviral vector delivery: 4~5 weeks
Steps to Order Ready-to-package Adenovirus:
1. Search our Adeno-Ready™ ORFs with NCBI accession #, gene symbol or GI number.
2. Identify adenoviral ORF clone and add the ready-to-package adenovirus into shopping cart.
3. Check out online.
4. We package the adenovirus and deliver adenoviral vectors at 1E+10~1E+11 PFU per ml.
Customized Service:
While the default promoter is CMV with standard deliverable, 0.2 ml at 1E+10 ~ 1E+11 PFU/ml, customized service is available with larger pack, different promoters (CAG, U6, H1, UBC or Synapsin) or reporters (GFP, mRFP, Luc, LacZ, etc). Please request a quote with us.
How to Search Ready-to-package Adenoviruses:
All the 37,430 ready to package adenoviruses can be readily identified via an online search using the Adeno-Ready™ ORF Search Tool. This convenient search engine allows you to find your gene of interest using NCBI Accession #, Gene Symbol, Gene ID, etc.
Find your gene of interest today! - Visit our Adeno_Ready ORF Search Engine
View our ready-to-package adenoviruses from the following categories:
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文献和实验一、技术背景： Adeasy 系统利用了腺病毒具有的高感染能力，可高度浓缩等优点，并破坏了腺病毒基因组中的早期基因E1，使之成为较为安全、高效的病毒载体。利用带有E1 基因的293 细胞作为包装细胞，通过倍比扩增，富集病毒颗粒，并通过CsCl 梯度离心，透析进行分离纯化。对绝大多数的细胞株可以达到近乎100％的感染效率。但需要指出的是Adeasy 同样具有所有病毒载体或转染试剂都不可避免的细胞毒性问题。 二、所需试剂： 1. 293 细胞；2. 重组好的腺病毒质粒；3. Pac
收集病毒。按步骤1收集细胞并准备病毒上清。通过Western blot和/或PCR鉴定重组腺病毒产生。 4、PCR鉴定重组病毒。取5μl病毒上清加入10μl蛋白酶K，55℃孵育1hr,再煮沸5min，离心后取1-2μl作PCR。 五、重组病毒扩增，纯化 1、75cm2方瓶中接种293细胞，至密度达到90%,加入适量病毒上清感染细胞。3-4d后，细胞几乎变圆，且有一半细胞漂浮，则收集所有细胞。约500g转速离心，弃上清。 2、以灭菌PBS重悬沉淀，反复冻融4次。4℃下7000g离心5min
1 腺病毒的包装，纯化和感染 技术背景：Adeasy 系统利用了腺病毒具有的高感染能力，可高度浓缩等优点， 并破坏了腺病毒基因组中的早期基因E1，使之成为较为安全、高效的病毒载体。 利用带有E1 基因的293 细胞作为包装细胞，通过倍比扩增，富集病毒颗粒，并 通过CsCl 梯度离心，透析进行分离纯化。对绝大多数的细胞株可以达到近乎100 ％的感染效率。但需要指出的是Adeasy 同样具有所有病毒载体或转染试剂都不 可避免的细胞毒性问题。 所需试剂：
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