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- ZYMO RESEARCH
- 美国
- D4015
- 2026年04月18日
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- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
ZR Plasmid Miniprep - Classic
- 库存：
大量
- 供应商：
简石生物
- 保存条件：
RT
HIGHLIGHTS
- High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
- Unique column design: Zero buffer retention and low (30 µl) elution volume.
DESCRIPTION
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
| • | For purification of high quality, endotoxin-free plasmid DNA for restriction endonuclease digestion, DNA sequencing, transformation, cloning, transfection, in vitro transcription reactions, etc. |
| • | Unique column design: zero buffer retention and low volume (30 µl) elution. |
| • | Innovative colored buffers for error-free visual identification of complete bacterial cell lysis and neutralization. |
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文献和实验v468 Chapter 9 自身启动子报告基因验证转录靶点
, whereasclones carrying the correct insert from the MMP7 promoterwill produce an amplicon of 1,028 bp (see Note 12 ). 4. Prepare plasmid DNA from a positive clone from the masterplateby using the miniprep kit from Qiagen and subject it tosequencing reaction
system, or interaction trap, is one of the most versatile methods available with which to identify and establish protein?protein interactions. The dual bait system is one adaptation of the classic approach. This system facilitates the simultaneous comparison
