- 询价
- ZYMO RESEARCH
- 美国
- D4036
- 2026年04月24日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
2年
- 英文名：
Zyppy Plasmid Miniprep Kit
- 库存：
50
- 供应商：
简石生物
- 保存条件：
常温
HIGHLIGHTS
- Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
- Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
DESCRIPTION
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
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文献和实验Procedure for GenEluteTM Plasmid Miniprep Kit（质粒小量提取）
upon the plasmid and culture density.For best yields,follow the instructions in the note below.Transfer the appropriate volume of the recombinant E.coli culture to a microcentrifuge tube and pellet cells at ≥12000 × g for 1 minute.Discard the supernatant. Note
Procedure for GenEluteTM Plasmid Miniprep Kit（质粒小量
volume of culture to use depends upon the plasmid and culture density. For best yields, follow the instructions in the note below. Transfer the appropriate volume of the recombinant E. coli culture to a microcentrifuge tube and pellet cells at ≥12000 × g
Materials Solution II: 0.2N NaOH/1% SDS Solution III: 3 M KOAc, pH4.8 RNAseA (DNAse free) 10 µg/mL Chloroform/Isoamyl alcohol (1/25 v/v) Isopropanol 70 % ethanol 13% PEG8000 Procedure 1) Culture 5 mL
