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R&D货号DVE00人血管内皮生长因子ELISA试剂盒136

11631389上海睿安生物
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  • 2025年11月24日
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    • 保存条件：

      2-8°C

    • 保质期：

      1年

    • 英文名：

      Human VEGF Quantikine ELISA Kit

    • 库存：

      100⁺盒

    • 供应商：

      上海睿安生物13611631389

    • 规格：

      1Kit/套/盒

    R&D货号DVE00人血管内皮生长因子ELISA试剂盒13611631389上海睿安生物

    Human VEGF Quantikine ELISA Kit

    Catalog #:DVE00

    Background:VEGF

    Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and the formation of antiparallel disulfide-linked dimers (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle (5, 6), hepatocytes (7), osteoblasts (8), neutrophils (9), macrophages (10), keratinocytes (11), brown adipose tissue (12), CD34+ stem cells (13), endothelial cells (14), fibroblasts, and vascular smooth muscle cells (15). VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M, and TNF-alpha (3, 4, 9, 16). VEGF isoforms are differentially expressed during development and in the adult (3). 

    VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR), and induce their homodimerization and autophosphorylation (3, 4, 7, 17, 18). These receptors have seven extracellular immunoglobulin-like domains and an intracellular split tyrosine kinase domain. They are expressed on vascular endothelial cells and a range of non-endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 also binds the semaphorin receptor, neuropilin-1, which promotes complex formation with VEGF R2 (19). 
    VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells (3, 4), thus regulating blood vessel density and size, but playing no role in determining vascular patterns. VEGF promotes bone formation through osteoblast and chondroblast recruitment and is also a monocyte chemoattractant (20-22). After birth, VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-vascular endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). In diseased tissues, VEGF promotes vascular permeability. It is thus thought to contribute to tumor metastasis by promoting both extravasation and tumor angiogenesis (23, 24). Various strategies have been employed therapeutically to antagonize VEGF-mediated tumor angiogenesis (25). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus(26).

    Long Name:Vascular Endothelial Growth Factor

    Entrez Gene IDs:7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

    Alternate Names:MVCD1;VAS;vascular endothelial growth factor A;Vascular permeability factor;Vasculotropin;VEGF;VEGFA;VEGF-A;VEGFMGC70609;VPF;VPFvascular endothelial growth factor

    Preparation and Storage:

    Shipping:The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Storage:Store the unopened product at 2-8°C.Do not use past expiration date.

    产品细节图片1

    产品细节图片2

    Product Summary:

    The Quantikine Human VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF165 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VEGF165 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human VEGF and recombinant human VEGF121 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human VEGF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF.

    Precision:

    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

    Cell Culture Supernates

    产品细节图片3产品细节图片4

    产品细节图片5

    产品种类丰富，质量稳定可靠.
    R&D总公司于1976年成立于美国，在美国NASDAQ上市(TECH)。公司一直致力于各种细胞因子及其相关分子的生产研发。R&D的产品覆盖面广，种类丰富，质量稳定可靠，其生产的各种ELISA试剂盒、重组因子及抗体以其卓越的品质赢得了世界各国科研及临床诊断机构的青睐。
    订购信息:

    货号产品描述规格
    DEP00Human Erythropoietin Quantikine IVD ELISA Kit人促红细胞生成素IVD ELISA试剂盒1Kit
    DVE00Human VEGF Quantikine ELISA Kit 人血管内皮生长因子ELISA试剂盒1Kit
    1324-WN-002Recombinant Mouse Wnt-3a重组鼠Wnt-3a2ug
    233-FB-025Recombinant Human FGF basic(146 aa)重组人FGF basic(146 aa)25ug
    240-B-002Recombinant Human TGF-beta 1重组人FGF basic(146 aa)2ug
    293-VE-010Recombinant Human VEGF 165重组人VEGF 16510ug
    314-BP-010Recombinant Human BMP-4重组人BMP-410ug
    338-AC-010Recombinant Human/Mouse/Rat Activin A重组人/小鼠/大鼠激活素A10ug
    338-AC-050Recombinant Human/Mouse/Rat Activin A重组人/小鼠/大鼠激活素A50ug
    355-BM-010Recombinant Human BMP-2重组人BMP-210ug

    产品细节图片6

    一、╳※试剂※╳:

    常规血清优势供应:VivaCell货号C04001-500血清、Gemini货号900-108血清、Gemini血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清
    培养基(包括无血清)、冻存液(包括无血清)，包括:友康Yocon
    Merck(Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)正式授权代理、博奥龙Biodragon独家总代理、EFL独家总代理、碧云天Beyotime代理
    ☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)
    分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen
    饲料类:Research Diets、Dyets
    二、╳※耗材※╳:
    洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶
    Bioplastics
    Thermo Fisher Scientific的很多产品线，包括旗下的:
    一、Nunc耗材
    二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)
    三、Nalgene耗材仪器
    四、FisherBrand耗材仪器(含各类盖玻片载玻片等)
    五、Lab_Serv耗材仪器
    六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)
    七、Abgene耗材
    畅销款:我们是常备有大量现货的
    可请随时咨询:万奎13611631389(可以请加微信)
    产品细节图片7
    ※╳√√✓✔√✓✔√✓✔上海睿安生物科技有限公司成立于2009年，专注于为生命科学和生物技术领域的客户提供优质的产品与服务。代理了多家欧美知名品牌，如美国Thermo Fisher耗材(包括旗下Nunc、QSP、Nalgene、FisherBrand、Lab_Serv耗材仪器、Finnpipette移液器、Abgene耗材，等等)、默克Merck-Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)、Zymo Research、洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材、博奥龙Biodragon独家总代理、EFL独家总代理、碧云天Beyotime代理、Eppendorf、友康Yocon、Research Diets高脂饲料，IBL、BioVendor、Mercodia、ShiBayaji代谢类产品、Axygen-Corning长期大量耗材备货、BioCaot畅销款长期备货，VivaCell血清、Gemini血清、Gibco血清，BI血清现货、ScienCell血清、Sigma血清、Moregate血清、Cegrogen血清、Pierce发光试剂、Fermentas、Invitrogen、Abcam、CST、BD、SAB、R&D systems、Beckman、Qiagen、BD Pharmingen、Biolegend、Jackson、Miltenyi、MP Biomedicals、Biotium更好的荧光试剂产品等品牌。睿安生物始终将“提供更好的产品和服务”作为企业发展的目标和努力的方向，并将通过优质的售前与售后服务，始终如一地为客户提供高质量的产品和服务，我们也希望能得到相关领域中更多老师和同仁们的指导和鞭策，为共创优秀品牌而努力奋斗！询订╳直线13611631389微信号

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    图标文献和实验
    该产品被引用文献

    R&D货号DVE00人血管内皮生长因子ELISA试剂盒13611631389上海睿安生物

    Human VEGF Quantikine ELISA Kit

    Catalog #:DVE00

    Background:VEGF

    Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and the formation of antiparallel disulfide-linked dimers (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle (5, 6), hepatocytes (7), osteoblasts (8), neutrophils (9), macrophages (10), keratinocytes (11), brown adipose tissue (12), CD34+ stem cells (13), endothelial cells (14), fibroblasts, and vascular smooth muscle cells (15). VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M, and TNF-alpha (3, 4, 9, 16). VEGF isoforms are differentially expressed during development and in the adult (3). 

    VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR), and induce their homodimerization and autophosphorylation (3, 4, 7, 17, 18). These receptors have seven extracellular immunoglobulin-like domains and an intracellular split tyrosine kinase domain. They are expressed on vascular endothelial cells and a range of non-endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 also binds the semaphorin receptor, neuropilin-1, which promotes complex formation with VEGF R2 (19). 
    VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells (3, 4), thus regulating blood vessel density and size, but playing no role in determining vascular patterns. VEGF promotes bone formation through osteoblast and chondroblast recruitment and is also a monocyte chemoattractant (20-22). After birth, VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-vascular endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). In diseased tissues, VEGF promotes vascular permeability. It is thus thought to contribute to tumor metastasis by promoting both extravasation and tumor angiogenesis (23, 24). Various strategies have been employed therapeutically to antagonize VEGF-mediated tumor angiogenesis (25). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus(26).

    Long Name:Vascular Endothelial Growth Factor

    Entrez Gene IDs:7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

    Alternate Names:MVCD1;VAS;vascular endothelial growth factor A;Vascular permeability factor;Vasculotropin;VEGF;VEGFA;VEGF-A;VEGFMGC70609;VPF;VPFvascular endothelial growth factor

    Preparation and Storage:

    Shipping:The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Storage:Store the unopened product at 2-8°C.Do not use past expiration date.

    引用文献图片1

    引用文献图片2

    Product Summary:

    The Quantikine Human VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF165 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human VEGF165 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human VEGF and recombinant human VEGF121 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human VEGF Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF.

    Precision:

    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

    Cell Culture Supernates

    引用文献图片3引用文献图片4

    引用文献图片5

    产品种类丰富，质量稳定可靠.
    R&D总公司于1976年成立于美国，在美国NASDAQ上市(TECH)。公司一直致力于各种细胞因子及其相关分子的生产研发。R&D的产品覆盖面广，种类丰富，质量稳定可靠，其生产的各种ELISA试剂盒、重组因子及抗体以其卓越的品质赢得了世界各国科研及临床诊断机构的青睐。
    订购信息:

    货号产品描述规格
    DEP00Human Erythropoietin Quantikine IVD ELISA Kit人促红细胞生成素IVD ELISA试剂盒1Kit
    DVE00Human VEGF Quantikine ELISA Kit 人血管内皮生长因子ELISA试剂盒1Kit
    1324-WN-002Recombinant Mouse Wnt-3a重组鼠Wnt-3a2ug
    233-FB-025Recombinant Human FGF basic(146 aa)重组人FGF basic(146 aa)25ug
    240-B-002Recombinant Human TGF-beta 1重组人FGF basic(146 aa)2ug
    293-VE-010Recombinant Human VEGF 165重组人VEGF 16510ug
    314-BP-010Recombinant Human BMP-4重组人BMP-410ug
    338-AC-010Recombinant Human/Mouse/Rat Activin A重组人/小鼠/大鼠激活素A10ug
    338-AC-050Recombinant Human/Mouse/Rat Activin A重组人/小鼠/大鼠激活素A50ug
    355-BM-010Recombinant Human BMP-2重组人BMP-210ug

    引用文献图片6

    一、╳※试剂※╳:

    常规血清优势供应:VivaCell货号C04001-500血清、Gemini货号900-108血清、Gemini血清、Gibco血清(10099-141澳洲血清、10270-106南美血清、16000-044血清)、BI血清BI货号04-001-1ACS、Sigma货号F8318-500ml澳洲胎牛血清、Sigma货号F8687澳洲胎牛血清、Sigma货号F2442-500ml特级胎牛血清、ScienCell货号0500澳洲胎牛血清、Cegrogen货号A0500-3010胎牛血清、Cegrogen货号B0500-5010新生牛血清、康源血清
    培养基(包括无血清)、冻存液(包括无血清)，包括:友康Yocon
    Merck(Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)正式授权代理、博奥龙Biodragon独家总代理、EFL独家总代理、碧云天Beyotime代理
    ☆☆☆☆☆☆Beckman货号A63881纯化磁珠Agencourt AMPure XP(60ml/瓶)/A63882(450ml/瓶)；Beckman货号B23317核酸片段筛选试剂盒Agencourt SPRIselect(5ml/瓶)
    分子类:Biotium货号41003的GelRed染料、货号31000的EvaGreen染料、Zymo Research、Qiagen
    饲料类:Research Diets、Dyets
    二、╳※耗材※╳:
    洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材/KirGen耗材、博麦迪(常规的)各类试剂瓶
    Bioplastics
    Thermo Fisher Scientific的很多产品线，包括旗下的:
    一、Nunc耗材
    二、QSP耗材(各类吸头/滤芯吸头、离心管、PCR管等)
    三、Nalgene耗材仪器
    四、FisherBrand耗材仪器(含各类盖玻片载玻片等)
    五、Lab_Serv耗材仪器
    六、Finnpipette移液器(如：F1系列、F2系列、F3系列等等各类型号的移液器)
    七、Abgene耗材
    畅销款:我们是常备有大量现货的
    可请随时咨询:万奎13611631389(可以请加微信)
    引用文献图片7
    ※╳√√✓✔√✓✔√✓✔上海睿安生物科技有限公司成立于2009年，专注于为生命科学和生物技术领域的客户提供优质的产品与服务。代理了多家欧美知名品牌，如美国Thermo Fisher耗材(包括旗下Nunc、QSP、Nalgene、FisherBrand、Lab_Serv耗材仪器、Finnpipette移液器、Abgene耗材，等等)、默克Merck-Millipore/Sigma/Roche/KaPa/Supelco/Avanti/Corning/Brand)、Zymo Research、洁特JET耗材(各类常规用实验耗材)、赛普Cellpro耗材、博奥龙Biodragon独家总代理、EFL独家总代理、碧云天Beyotime代理、Eppendorf、友康Yocon、Research Diets高脂饲料，IBL、BioVendor、Mercodia、ShiBayaji代谢类产品、Axygen-Corning长期大量耗材备货、BioCaot畅销款长期备货，VivaCell血清、Gemini血清、Gibco血清，BI血清现货、ScienCell血清、Sigma血清、Moregate血清、Cegrogen血清、Pierce发光试剂、Fermentas、Invitrogen、Abcam、CST、BD、SAB、R&D systems、Beckman、Qiagen、BD Pharmingen、Biolegend、Jackson、Miltenyi、MP Biomedicals、Biotium更好的荧光试剂产品等品牌。睿安生物始终将“提供更好的产品和服务”作为企业发展的目标和努力的方向，并将通过优质的售前与售后服务，始终如一地为客户提供高质量的产品和服务，我们也希望能得到相关领域中更多老师和同仁们的指导和鞭策，为共创优秀品牌而努力奋斗！询订╳直线13611631389微信号
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