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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
LC3 (APG8A) (AA 91-117), (Asp106) 抗体
- 规格:
0.1ml/0.2ml
LC3 (APG8A)
抗原表位
其他选择 AA 91-117, Asp106
适用
其他选择 人
宿主
其他选择 兔
克隆类型 (克隆位点)
多克隆 (RB13262)
应用范围
其他选择 免疫萤光(IF), Western Blotting (WB)
Pubmed 2个引用可参考
规格 400 μL
发货至 中国 (更改)
发货时间 9至13个工作日
产品编号 yb388467
产品细节 LC3 (APG8A) (AA 91-117), (Asp106) 抗体
免疫原 This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-117 AA from human LC3.
克隆位点 RB13262
亚型 Ig
特异性 This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92~121 amino acids from the center region of human LC3 (APG8a).
预测反应 Cow (Bovine),Mouse (Murine),Rat (Rattus)
纯化方法 This antibody is purified through a protein A column, followed by peptide affinity purification.
目标详细情况LC3 (APG8A) (AA 91-117), (Asp106) 抗体
背景 Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate
分子量 14272 DA
基因ID 84557
UniProt Q9H492
研究领域 Cell Structure, Autophagy
使用细节 LC3 (APG8A) (AA 91-117), (Asp106) 抗体
应用备注 IF = 1:100, WB = 1:1000
限制 仅限研究用
贮存及处理LC3 (APG8A) (AA 91-117), (Asp106) 抗体
状态 Liquid
浓度 0.33 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
有效期 6 months
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文献和实验【原创】转载Western Blot 内参选择攻略(美季生物技术有限公司)http://www.majorbio.cn/NewsInfo.asp?id=289
的检测是有很大的影响,买抗体前要好好熟悉自己的目的蛋白,很多抗体杂不出条带其实未必是抗体质量的问题。很多公司的内参抗体是用抗原片段制备的,不同的公司选择的片段不一样。以最常用的beta-actin为例,有的公司选择N-端,有的选择C-端。选用N-端Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys 16aa作为抗原制备抗体(单抗和多抗)的占大多数,主要有santa cruz(Cat# sc-69879),sigma(Cat
:LC3抗体对LC3-II有更高的亲和力,会造成假阳性。方法2和3需结合使用,同时需考虑溶酶体活性的影响。) 4)检测长寿蛋白的批量降解:非特异 5)MDC(Monodansylcadaverine,单丹磺酰尸胺)染色:包括自噬体,所有酸性液泡都被染色,故属于非特异性的。 6)CellTrackerTM Green染色:主要用于双染色,但其能染所有的液泡,故也属于非特异性的。 在研究自噬相关蛋白时,需对其进行定位。由于自噬体与溶酶体、线粒体
中。5. 用无菌 PBS 将上述锥形试管填满(至 50 mL)。介绍样品制备。6. 洗涤。在室温、低速制动条件下,以 300 X g 离心分离试管 10 分钟。7. 裂解红细胞(BD 产品目录号:555899,10 x 溶液。在蒸馏水中使用 1 x 溶液。室温孵育 15 分钟。)8. 将 1x106 PBMC 重悬于 20 μL 染色缓冲液中(PBS 含有 5% 正常人血清)。9. 加入抗体:CD117-BV421 (c-kit)、CD7-BV510、CD57- BV605、CD8-BV650
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