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- promega
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- L2015
- 2026年01月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
1ml (20 × 50µl)
| L2015 | Single-use HB101 Competent Cells | 1ml (20 × 50µl) | 1,771 |
| L2080 | TNT® SP6 Quick Coupled Transcription/Translation System | 40 reactions | 4,819 |
| L2081 | TNT® SP6 Quick Coupled Transcription/Translation System, Trial Size | 5 reactions | 1,320 |
| L3002 | Single Step (KRX) Competent Cells | 20 × 50µl | 2,501 |
| L3260 | TNT® SP6 High-Yield Protein Expression System | 40 reactions | 5,446 |
| L3261 | TNT® SP6 High-Yield Protein Expression System | 10 reactions | 1,694 |
| L4130 | TNT® SP6 Coupled Wheat Germ Extract System | 40 reactions | 4,433 |
| L4140 | TNT® T7 Coupled Wheat Germ Extract System | 40 reactions | 4,433 |
| L4151 | Rabbit Reticulocyte Lysate, Untreated | 1ml | 1,149 |
| L4330 | Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System | 24 reactions | 1,793 |
| L4380 | Wheat Germ Extract | 5 × 200µl | 1,591 |
| L4461 | Amino Acid Mixture, Complete | 175µl | 519 |
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文献和实验Transformation of HB-101 Competent Cells
实验步骤 1. Thaw tubes of competent cells on ice, occasionally inverting gently to mix. 2. Pre-cool Falcon 2059 tubes on ice. 3. Aliquot 30 ul cells into each tube. 4. Add up to 10 ul DNA in TE or ligation
works pretty well if incubated for 20-30min on ice but I have not optimized it. For plasmids I see no reason to use any other bugs than HB101 which works extremely well with this procedure. -Spin the cells as gently as possible, at 2Krpm for 5min at 4℃
实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
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