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- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
5mg
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文献和实验. Thomson, M. Newman, J. S. Parker, E. M. Morin-Kensicki, T. Wright, S. M. Hammond, Extensive post-transcriptional regulation of microRNAs and its implications for cancer. Genes Dev. 20, 2202–2207 (2006).[Abstract/Free Full Text] 18. G. A. Calin, C. M
, Germany) according to the manufacturer's instruction with minor modifications [11]. Purity was routinely >98% as assessed by fluorescence analysis.Cell stimulationPurified CD19+ B cells were cultured in Iscove Dulbecco's Modified Medium (IDMM; Bio
QUALITATIVE ANALYSIS OF DNA FRAGMENTATION BY AGAROSE GEL ELECTROPHORESIS
cells at 200xg at 4°C for 10 min. 3. Transfer supernatants carefully in new tubes labeled S (supernatant). 4. Add to the pellet in tubes B 0.5 ml of TTE solution and vortex vigorously. This procedure allows the release of fragmented chromatin
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