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文献和实验Transformation of HB-101 Competent Cells
实验步骤 1. Thaw tubes of competent cells on ice, occasionally inverting gently to mix. 2. Pre-cool Falcon 2059 tubes on ice. 3. Aliquot 30 ul cells into each tube. 4. Add up to 10 ul DNA in TE or ligation
实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
of desired strain (see below for possibilities) for 2 hours in 5ml of LB. Shake vigorously at 37 ℃. (300rpm in orbital shaker). -Transfer to 100ml fresh LB broth. Shake for a further 2-3 hours till OD550 = 0.5. (DH1, JM101 takes ~2 hours; HB101, DH5aF
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