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文献和实验60ml Glycerin To make: mix, pH down to 5.8 with diluted (about 0.2M) aectic acid, sterile filter, keep at 4 ℃. TfbII 500ml 10.47g Mops, dissolve in 300ml distilled water, adjust pH to pH7.0 with 10M NaOH
Transformation of E. coli by Electroporation
and then pressing the UP voltage control button until the voltage reading is 5 to 10 volts higher than the desired discharge voltage. For E. coli , the standard conditions are 2.4 kv, which means setting the Pulse Control unit to 405 volts (400 volts is the desired
Transformation Ultra-Competent E. coli
Transformation "Ultra-Competent" E. coli (Inoue Method) Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96 :23-28. Citation Abstract Procedure
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