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- 2026年01月07日
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文献和实验Eukaryotes repair DNA double-strand breaks (DSBs) by homologous recombination (HR) or by nonhomologous end-joining (NHEJ). DSBs are a natural consequence of DNA metabolism, occurring, for example, during DNA replication and meiosis. DSBs
Monitoring I-SceI-Induced Double-Strand Break Repair in DT40 Cells
. The combination of this genetic tractability with the I-SceI-induced double-strand break (DSB) repair assay, pioneered by the laboratory of Maria Jasin, has proven particularly powerful for dissecting the pathways of DSB repair in a vertebrate setting.
-based reporters with recognition sites for the rare-cutting endonuclease I-SceI. One set of reporters is used to measure DSB repair events that require access to homology: homology-directed repair, homology-directed repair that requires the removal
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