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文献和实验Opinion in Biotechnology 6:30-36,1995 n) Make-your-own TA-cloning vector - can use plasmid pDK101 [ATCC 77406], a derivative of plasmid pGEM[R]5fZ(+). Digest with the endonuclease XcmI which give the overhangs for cloning. Another way is to digest
-8, 1992, Rashtchian, Current Opinion in Biotechnology 6:30-36, 1995 n) Make-your-own TA-cloning vector - can use plasmid pDK101 [ATCC 77406], a derivative of plasmid pGEM[R]5fZ(+). Digest with the endonuclease XcmI which give the overhangs
, Biotechniques 13(4)515-8,1992,Rashtchian,Current Opinion in Biotechnology 6:30-36,1995 n) Make-your-own TA-cloning vector - can use plasmid pDK101 [ATCC 77406], a derivative of plasmid pGEM[R]5fZ( ). Digest with the endonuclease XcmI
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