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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 库存:
大量
- 供应商:
上海善然生物科技有限公司
- 规格:
1,000 assays
| E4550 | Luciferase 1000 Assay System | 1,000 assays | 5,461 |
| E4611 | pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector | 20μg | 3,570 |
| E4651 | pGL4.52[luc2P/STAT5RE/Hygro] Vector | 20μg | 3,570 |
| E4720 | Beta-Glo® Assay System | 10ml | 887 |
| E4740 | Beta-Glo® Assay System | 100ml | 6,372 |
| E4780 | Beta-Glo® Assay System | 10 × 100ml | 40,136 |
| E4881 | Transfection Carrier DNA | 5 × 20μg | 1,522 |
| E4910 | Chroma-Glo™ Luciferase Assay System | 10ml | 1,634 |
| E4920 | Chroma-Glo™ Luciferase Assay System | 100ml | 9,901 |
| E4981 | ViaFect™ Transfection Reagent | 0.75ml | 3,327 |
| E4982 | ViaFect™ Transfection Reagent | 2x 0.75ml | 5,595 |
| E4983 | ViaFect™ Transfection Reagent | 0.2ml | 951 |
| E5011 | pGL4.53[luc2/PGK] Vector | 20ug | 2,947 |
| E5061 | pGL4.54[luc2/TK] Vector | 21ug | 2,947 |
| E5750 | Erase-a-Base® System (minus vectors & bacterial strain) | 1 system | 8,611 |
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文献和实验The Split Luciferase Complementation Assay
A split luciferase complementation assay to study protein–protein interactions within Arabidopsis protoplasts in 96-well plates is described in this protocol. Two proteins of interest, a bait and prey, which are genetically fused to amino
superskyfly 我自己都觉得我自己很牛,千百人都做出来的试验,我就是做不出来。质粒用的是Promega p4.32-NF-kB-Luc, 转染用的是Invitrogen的Lipofactamin, 激活用的是Sigma生产的PMA和PHA-P,Assay用的是Promega dual,结果加了PMA和PHA的和不加的读数几乎没有区别。加了compound的和不加的也没有区别。 用过PC3, Jurkat, A549,HCT116都是一样。
Measuring HIV Neutralization in a Luciferase Reporter Gene Assay
.Moreover, these assays may be used with molecularly cloned Env-pseudotyped viruses for greater reagent stability and traceability.A luciferase (Luc) reporter gene assay performed in TZM-bl (JC53bl-13) cells was recently optimized and many of its performance parameters
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