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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
50ml1XCellLysisBuffer+100mlLuciferaseAssayBuffer+1mlx10Luciferasesubstrate
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文献和实验Improvements in the Application of Firefly Luciferase Assays
The firefly luciferase-based assay differs from most familiar enzyme-based determinations. Most enzyme assays are based either on the production of a product or the disappearance of a substrate. Usually the compound measured is stable
to shear DNA to lengths between 200 and 1000 basepairs being sure to keep samples ice cold. 5,� Centrifuge samples (part A, step 7) for 10 minutes at 13,000 rpm at 4�C, and detected the OD260 of the lysates. 6, Sonicated lysates
Measuring HIV Neutralization in a Luciferase Reporter Gene Assay
.Moreover, these assays may be used with molecularly cloned Env-pseudotyped viruses for greater reagent stability and traceability.A luciferase (Luc) reporter gene assay performed in TZM-bl (JC53bl-13) cells was recently optimized and many of its performance parameters
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