多聚-D-赖氨酸(Sigma代理)P6407-5MG

Frequently Asked Questions

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Components

分子量 >30,000 的聚赖氨酸适用于促进细胞粘附到固体基质。

Application

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm². Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Caution

无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在​-20°C条件下。

Preparation Note

分子量基于粘度。