Plasmid DNA transfection reage

TaKaRa RNAiso Reagent

本制品是一种快速方便的总RNA提取试剂，可以从动物组织、植物材料、各种微生物、培养细胞等中提取总RNA。样品在RNAiso Reagent中能够充分被裂解，在加入氯仿离心后，溶液会形成上清层、中间层和有机层（下层)，RNA分布在上清层中，收集上清层后，经异丙醇沉淀便可以回收得到总RNA。RNAiso Reagent具有很强的广谱性，可以适用于各种样品的总RNA提取，提取过程方便快速，整个操作在一小时内便可以完成。经本制品提取的总RNA纯度高，基本不含蛋白质及基因组 DNA，提取的RNA可以直接

Cationic Liposome-Mediated Transfection with Lipofectin Reagent

(2 ,3 ). Some encouraging DNA-delivery results have been obtained; however, the methodology has had some fundamental diffkulties (4 –8 ). Chief among these is that liposomes do not generally fuse with the target cell surface, but are taken up phagocytically

DNA Transfection

lines and subsequent measurement of reporter enzyme activity in cell extracts. Following ligation of the chosen promotor sequence to the reporter enzyme coding region in a suitable plasmid vector, DNA is purified and transfected into an appropriate cell